Wako 129-02541賴氨酰肽鏈內切酶 Lysyl Endopeptidase

Wako 129-02541賴氨酰肽鏈內切酶 Lysyl Endopeptidase





英文名:Lysyl Endopeptidase?

規格:1vial (10AU) /1vial (2AU)

Lysyl Endopeptidase, originally isolated from the soil bacterium discovered by Masaki, et al. cleaves specifically the peptide bonds at the carboxy-terminal side of Lysine residues and S-aminoethylcysteine residues with a high degree of specificity, making it a valuable tool for protein sequence analysis and for enzymatic synthesis of Lys-X compounds. An added feature of Lysyl Endopeptidase is its ability to retain complete activity after incubation in 4M urea or in 0.1% SDS solution for up to 6 hours at 30 degrees C.
Appearance: Lyophilized form containing ca. 10% Tris-HCl buffer, pH 8.

Activity: Shown on each label

Molecular Weight: 27,000 (gel filtration); 30,000 (SDS electrophoresis)

Solubility: Soluble in water or buffer solution.

Stability: Stable at 4 degrees C, when dissolved in buffer of pH 5~12. Stable at 30 degrees C in the range of pH 6~11, but unstable at 50 degrees C or higher.

Optimal pH: 9.0 -9.5 (Amidase activity)

Isoelectric point: 6.9~7.0

Substrate specificity:
Hydrolysable substrate … Tos-Lys-Ome, Bz-Lys-NH2, Bz-Lys-pNA, Lys-pNA
Unhydrolysable substrate … Bz-Arg-NH2, Bz-Arg-pNa, Arg-pNA

Inhibitors: DFP, PMSF, TLCK

Unit definition: One amidase unit (AU) is the amount of enzyme, which will produce 1 micromole of p-Nitroaniline per minute at 30 degrees C, pH 9.5.
Source: Bacteria

Originally, the source of this product was indicated as “Achromobacter lyticus” based on the physiological and morphological properties of the bacteria. However, we confirmed that the 16SrDNA sequence was highly homologous to that of Lysobacter.?