蘭格組織灌流用基本型蠕動泵 BT100-2J

蘭格組織灌流用基本型蠕動泵 BT100-2J

BT100-2J/BT100-1L/Lead-2醫學院及科研機構的藥理、生理實驗手段之一,它提供帶氧氣輸入的數控恒溫環境,從而保持離體組織器官的生理活性,使相關實驗順利進行。適合用于血管、平滑肌、心肌、冠狀動脈、大腸等組織器官的生理學以及藥理學研究。

簡介:?蘭格蠕動泵BT100-2J可安裝多種泵頭,能提供0.0002~380ml/min(單管)的流量范圍。轉速可手動調節,也可通過外控接口進行自動控制。采用手感薄膜按鍵,操作簡便直觀。

技術指標:

轉速范圍: 0.1~100rpm? 正反轉可逆

轉速分辨率: 0.1 rpm

調節方式: 薄膜按鍵連續調節

顯示方式: 3位LED數碼轉速顯示

外控功能: 外控啟停、外控方向,帶隔離,TTL電平輸入;0-5V/10V、4-20mA、0-10KHz速度控制輸入

適用電源: AC 90V-260V? 50Hz/60Hz

消耗功率: <30W

工作環境: ?環境溫度0 ~ 40℃? 相對濕度<80%

外形尺寸(長×寬×高): 232×142×149(mm)

驅動器重量: 2.3Kg

IP等級: IP31

功能特點:

可安裝多種泵頭: YZ1515x、YZ2515x、YZⅡ15、YZⅡ25、DG-1、DG-2

MAX 鍵: 實現填充排空功能(全速)

轉速可手動調節,也可通過外控接口進行控制

掉電記憶功能: 重新上電后,按照上次掉電時的狀態進行工作

通訊功能: RS485通訊

具有良好的性價比

不適于戶外使用

驅動器 適用泵頭 適用軟管 參考流量范圍(mL/min) 通道數 重量(kg)
BT100-2J YZ1515X???? YZⅡ15 13# 14# 19# 16# 25# 17#18# 0.07 – 380 1 3.8
YZ2515X??? YZⅡ25 15# 1.7 – 150 1 3.8
DG-1(6滾輪) 壁厚0.8 – 1mm

內徑 < 3.17mm

0.00025 – 48 1 3.5
DG-1 (10滾輪) 0.0002 – 32 1 3.5
DG-2(6滾輪) 單通道0.00025 – 48 2 3.6
DG-2(10滾輪) 單通道

0.0002 – 32

2 3.6

美國Kimble微量電動組織勻漿器(馬達+研磨槌)

美國Kimble微量電動組織勻漿器(馬達+研磨槌)

微量電動組織勻漿器電源是兩個AA干電池,可直接用在離心管里勻漿,配上不同的槌和管便可實現從0.5ml至50ml的組織勻漿;勻漿快速,便用輕便,操作簡單,無需特別維護。提供無菌包裝,無DNA酶和RNA酶,無致熱源的一次性使用槌;也有可反復使用的聚丙烯(PP)材料槌和不銹鋼(SS)槌。

產品編號 產品描述 產品包裝  
微量電動組織勻漿器0.5ml套裝
749540-0000 馬達 1臺  
749521-0500 槌;規格0.5ml;制作材料PP 100/  
749516-0500 槌;規格0.5ml;制作材料SS 1個/箱  
MCT-060-C AXYGEN?0.5ml離心管 1000支/袋  
微量電動組織勻漿器1.5ml套裝

749540-0000

馬達

1臺

749521-1500

槌;規格1.5ml;制作材料PP

100/

749515-0000

槌;規格1.5ml;制作材料SS

1個/箱

MCT-150-C

AXYGEN?1.5ml離心管(耐沸騰)

500個/包

微量電動組織勻漿器15ml套裝

749540-0000

馬達

1臺

749515-1500

槌;規格15ml;制作材料SS

1/

188261

15ML離心管

50個/包

微量電動組織勻漿器50ml套裝

749540-0000

馬達

1臺

749515-5000

槌;規格50ml;制作材料SS

1/

227270

Greiner?50ML離心管

25個/包

無菌獨立包裝?(非常規)75折

749521-0590

槌;規格:0.5ml;制作材料:?PP一次性

100個/包

749521-1590

槌;規格:1.5ml;制作材料:??PP一次性

100個/包

749520-0090

槌;1.5ml獨立包裝帶離心管PP一次性

100套/箱

749520-0590

槌;0.5ml獨立包裝帶離心管PP一次性

100套/箱

CryoScarless DMSO-Free 無DMSO干細胞凍存液

CryoScarless DMSO-Free 無DMSO干細胞凍存液

品牌:Bio Verde
儲存條件:+4℃

高存活率細胞凍存液

CryoScarless??DMSO-Free

CryoScarless??DMSO-Free 是一款不含蛋白和 DMSO,適用于多種細胞的細胞凍存液。各種細胞在解凍后具有很高的培養活率,且維持了干細胞的多分化性(未分化狀態)。

※ 本產品僅供科研,不可用于科研外其他用途。

關于含有DMSO凍存液的問題

DMSO作為普通細胞冷凍保護成分常被添加到凍存液中,但同時也是對人體有害的成分,且極易被皮膚吸收。除具有細胞毒性外,如同下列論文所述,是影響細胞分化的因子之一。因此,為獲得更精確的實驗結果,無DMSO的細胞保存是必須的。

● 使用DMSO將人骨髓白血病細胞株 HL-60 分化為粒細胞。1

● 使用DMSO將小鼠間充質干細胞分化為心肌細胞。?2

● 使用含有DMSD的凍存液凍存人ES細胞發現未分化標記Oct-4的表達降低。?3

 

參考文獻

1. Jiang, G.,?et al., Int. Immunopharmacol.,?6?(7), 1204~1213 (2006).

2. Young, D. A.,?et al., Biochem. Biophys. Res. Commun.,?322?(3), 759~765 (2004).

3. Katkov, I. I.,?et al., Cryobiology,?53?(2), 194~205 (2006).

◆特點

● 不含血清和蛋白,因此不受白蛋白和球蛋白等蛋白的影響。

● 不受DMSO毒性和蛋白影響,可安全且高活率地凍存細胞。

● 大部分細胞在凍存后復蘇,存活率可達90%以上。

● 已有人/小鼠的正常細胞、腫瘤細胞株、干細胞等各種細胞的凍存應用。

● 凍存后可維持干細胞的分化性能。

● 同樣適用于無血清培養。

● 經無菌測試確認不含細菌、真菌與支原體污染。

● 本產品可在4°C下長期保存。(有效期2年)。

※ 使用臺盼藍處理CryoScarless保存的細胞時,請務必清洗細胞。

◆應用實例1

大鼠間充質干細胞分化能力

311585622803.jpg

確認使用本產品凍存的大鼠間充質干細胞在復蘇后的分化能力,并與未凍存組以及10% DMSO保存的細胞進行比較。結果顯示,使用本產品凍存的細胞皆維持良好的分化性能。

大鼠間充干細胞的存活率

2.jpg

檢測使用本產品凍存(1周)的大鼠間充質干細胞在復蘇后的存活率以及 6 h后的存活率,并與10% DMSO保存的情況進行比較。本產品無論是在剛復蘇(0 h),還是貼壁后(6 h)皆顯示更高的存活率。

◆應用實例2

使用 CryoScarless??DMSO-Free 簡單凍存后的小鼠 ES 細胞

實驗方法:

將小鼠 ES 細胞(FKHR+/+)在明膠包被的96孔板上于37°C培養48 h后,進行簡單地凍存。吸走培養基并用PBS清洗,然后分別添加10%DMSO/培養基,或CryoScarless??DMSO-Free,在-80°C下保存。24 h后迅速復蘇,添加加熱至37°C的培養基,在37°C下繼續培養。6 h后使用光學顯微鏡觀察相襯圖像。

image.png

結果:

可觀察到在10%DMSO/培養基中保存的小鼠ES細胞在復蘇后活細胞減少(左圖),而在 CryoScarless??DMSO-Free 中保存的小鼠ES細胞狀態得到改善,可觀察到大部分的活細胞。

討論:

在對多個轉基因克隆的培養板一起進行凍存時(簡易凍存),使用CryoScarless??DMSO-Free 更加方便。利用 CryoScarless??DMSO-Free 進行凍存,存活細胞更多,更易于未分化小鼠ES細胞的傳代培養(在一定細胞密度下每2天傳代一次),并可順利進行分化實驗。

數據提供

◆應用實例3

對于人牙髓來源間充質干細胞的體內實驗,CryoScarless??DMSO-Free 擁有更出色的凍存能力

日本牙科大學生命牙學部 發育、再生醫科講座 中原貴教授

生命牙科學講座 望月 真衣 助教

Establishment of xenogeneic serum-free culture methods for handling human dental pulp stem cells using clinically oriented in-vitro and in-vivo conditions.

Stem Cell Research & Therapy,,?9:25, (2015).

 

背景

由于再生醫學不斷普及,因此急需確立可安全地培養從患者獲得的間充質干細胞的方法。近年來,通過牙科治療從拔牙的牙組織(牙髓)中獲得的間充質干細胞,顯示比骨髓干細胞高3~4倍的高增殖性,且沒有伴隨染色體異常的細胞變化。而且牙髓干細胞擁有與骨髓干細胞相同的多分化性能。由于在體外培養可大量增殖,靈活利用低癌變和成瘤風險的牙髓干細胞,對于確保醫療安全性的再生醫學來說是一個極具吸引力的選擇。

作為再生醫學中必不可少的”細胞凍存”,已經證明CryoScarless??DMSO-Free(BioVerde公司)是一款優秀的細胞凍存液。換言之,使用該細胞凍存液凍存的人牙髓干細胞擁有與未經凍存培養的牙髓干細胞相同的增殖能與多分化能力,且未發現染色體異常。

本文著眼于牙髓干細胞活躍的增殖能力,并證實了CryoScarless??DMSO-Free不會影響細胞增殖能力。

方法及結果

從20~37歲成人拔下來的智齒分離牙髓細胞并進行無血清培養,培養至傳代數為1時使用CryoScarless??DMSO-Free凍存。3個月后再次培養凍存的細胞,傳代數到3時進行以下的細胞評價。同時,對未經凍存的牙髓細胞也進行了相同的評價。

使用相差顯微鏡觀察細胞形態,確認紡錘形的成纖維細胞形態沒有發生變化(圖-1)。根據增殖曲線分析的結果,經過12天的培養,呈現一致的細胞增殖模式,兩者間在統計學上的沒有顯著差異(圖-2)。另外,通過Bromodeoxyuridine(BrdU)進行細胞分裂能力分析的結果顯示,可觀察到對數生長期內大量的BrdU陽性細胞,細胞數在統計學上沒有顯著差異(圖-3)。

311585623092.jpg

圖-1 人牙髓干細胞的相差顯微鏡圖像

無論CryoScarless??DMSO-Free凍存(左)還是未經凍存(右)的細胞都呈現出相同的成纖維細胞樣形態。

311585623148.jpg

圖-2 人牙髓干細胞增殖曲線分析

細胞培養期間,未發現兩者在統計學上的顯著差異。

311585623232.jpg

圖-3 人牙髓干細胞在對數生長期內的BrdU分析

可觀察到大部分細胞攝入BraU(綠)(左),未發現兩者的BraU陽性細胞數在統計學上的顯著差異(右)。

結論

除了本文中報告的細胞增殖能力評價之外,使用CryoScarless??DMSO-Free凍存的牙髓干細胞不僅維持了成骨細胞、脂肪細胞、軟骨細胞的多分化能力,也能維持向免疫缺陷小鼠的皮下移植并引起硬組織形成的能力。并且該產品并不影響干細胞的特性。

另外,值得注意的是,使用CryoScarless??DMSO-Free凍存的牙髓干細胞即使長期培養至傳代數為10后仍然保持染色體的二倍體和正常核型,大量培養的細胞中也沒有發現染色體異常。

在再生醫學中必不可少的”細胞凍存”中,作為冷凍保護劑使用的Dimethyl sulfoxide(DMSO)可能會有細胞毒性和引起意外的細胞分化,因此,我們十分期待能夠確立一個像該產品一樣的無DMSO的細胞凍存體系。

本報道中使用易于獲得的牙髓來源間充質干細胞,研究了CryoScarless??DMSO-Free對干細胞特性的影響,并確認了使用該產品凍存的細胞與未經凍存的牙髓干細胞擁有相同的細胞特性。因此,CryoScarless??DMSO-Free在間充質干細胞基礎研究以及臨床前研究,甚至是將來的再生醫學領域中,有望成為一款可以提供安全且高預測性數據和治療成果的細胞凍存液。

◆實際應用的細胞和凍存效果

細胞株 存活率(%)*
L929 小鼠成纖維細胞 97.5 ± 1.2
MG63 人骨肉瘤細胞 93.1 ± 2.3
HT1080 人纖維肉瘤細胞 90.2 ± 4.3
Colon26 小鼠結腸癌細胞 92.3 ± 2.3
B16F1 小鼠黑素瘤細胞 94.2 ± 0.6
KB 人口腔癌細胞 91.8 ± 0.9
Caco2 人結腸癌細胞 93.7 ± 1.9
MC3T3 小鼠成骨細胞 94.4 ± 0.5
Jurkat E6-1 人白血病細胞 88.4 ± 2.5
HUVEC 人臍靜脈內皮細胞 89.9 ± 0.4
HCAEC 人冠狀動脈內皮細胞 90.1 ± 1.6
MEF 小鼠胎兒成纖維細胞 94.4 ± 0.8
hACh 人軟骨細胞 93.5 ± 0.7

* -80°C凍存3個月,復蘇后24 h

◆操作方法概要

1. 將培養細胞放入離心管,離心并去掉上清。

2. 按照5×105~5×106?cells/mL添加本產品,并制成細胞懸液,每個凍存管分裝1 mL。

3. 放入-80℃超低溫冰箱中凍存。

4. 將在上一步中保存的細胞放入37°C恒溫水浴槽中,快速晃動使細胞解凍,融化后放入適當的培養基(10 mL)中并離心清洗。

※長期保存請置于液氮罐中保存。

◆產品列表

產品編號 產品名稱 包裝
CPL-A1 CryoScarless DMSO-Free
無DMSO干細胞凍存液
100 mL

細胞凍存液

 

品牌 貨號 名稱 規格 應用
LYMPHOTEC 306-95921 BAMBANKER??Direct
BAMBANKER直接凍存液
20 mL 常見的小鼠、大鼠、狗、靈長類等細胞系
306-14684 BAMBANKER?

BAMBANKER凍存液

20 mL×5 外周血細胞,及常見的小鼠、大鼠、靈長類等細胞系
302-14681 120 mL
Wako 195-16031 StemSure??Freezing Medium
StemSure干細胞凍存液
100 mL 人iPS、小鼠ES、及常見的小鼠、大鼠、人、狗等細胞系
039-23511 CultureSure Freezing Medium
CultureSure通用動物細胞凍存液
100 mL 常見的小鼠、大鼠、狗等細胞系
Stemgent 01-0013-50 CryoStem™ Freezing Medium

干細胞凍存液

50 mL 人ES/iPS/間充質干細胞
ReproCell RCHEFM001 Freezing medium for human ES/iPS cells
靈長類ES/iPS胞凍存液
25 mL 靈長類ES/iPS細胞
Funakoshi CPL-A1 CryoScarless DMSO-Free
靈長類ES/iPS玻璃化細胞凍存液
100 mL 間充質干細胞,及各種細胞玻璃化凍存
VPL-A1 Stem Cell Keep
玻璃化細胞凍存液
20 mL ES/iPS細胞
Cosmo Bio KOJ-COS-CFM01 COS banker [Cell Freezing Medium]、(chemical difined)
COS banker【細胞凍存液】

(化學成分明確)

120 mL 貼壁細胞、原代細胞、血液細胞、雜交瘤細胞等
KOJ-COS-CFM02 COS banker II
COS banker II【細胞凍存液】
120 mL 外周血淋巴細胞等COS banker適用細胞株

 

 

◆凍存液成分和特點比較

 

品牌 名稱 DMSO 血清 化學成分限定 程序降溫
LYMPHOTEC BAMBANKER Direct × × ×
BAMBANKER × × ×
Wako StemSure??Freezing Medium × × ×
CultureSure Freezing Medium × × ×
Stemgent CryoStem™ Freezing Medium ×
ReproCell Freezing medium for human ES/iPS cells × × ×
Funakoshi CryoScarless DMSO-Free × × ×
Stem Cell Keep × × ×
Cosmo Bio COS banker [Cell Freezing Medium] (chemical difined) × ×
COS banker II × ×

 

意大利 HANNA哈納 HI93715-01氨氮試劑

意大利 HANNA哈納 HI93715-01氨氮試劑

HANNA哈納代理

意大利HANNA哈納HI93715-01氨氮試劑,適用于范圍:0.00 to 9.99 ppm(mg/L) NH3-N;使用預制試劑,化學品的量和處理時間也被小化,測試準備時間大大縮短,并且沒有耗時的試劑制備程序或玻璃器皿清潔;
HI93715-01 定制氨氮試劑 預測次數 100次

HI93715-03 定制氨氮試劑 預測次數 300次

定制氨氮(MR)試劑,適用于范圍:0.00 to 9.99 ppm(mg/L) NH3-N;方便快捷實現氨氮快速準確的比色測量,測定方法符合ASTM D1426-92, Nessler?鈉氏試劑比色法;
HI93715-01?定制氨氮試劑 ?預測次數?100次;HI93715-03?定制氨氮試劑 ?預測次數?300次

使用預制試劑,化學品的量和處理時間也被小化,測試準備時間大大縮短,并且沒有耗時的試劑制備程序或玻璃器皿清潔;

預制高質量氨氮測量試劑,經過嚴苛認證各種量具和容器,在符合要求恒溫恒濕環境下精心配制而成,并可追溯到NIST標準參考物質(SRM)

供應意大利HANNA哈納HI93715-01氨氮試劑

試劑型號 預測次數 推薦光度測定儀
HI93715-01 預測次數:100 HI97715、HI96715、HI833XX系列等氨氮光度測定儀
HI937015-03 預測次數:300

高品質氨氮預制試劑,適用測量范圍:0.00 to 9.99 ppm(mg/L) NH3-N;遵循ASTM D1426-92, Nessler?鈉氏試劑比色法;,您能方便實現快速準確的比色測量。

分別將4HI93715A-0HI93715B-0 預制氨氮試劑加入待測水樣中【詳見操作使用手冊】,含有氨樣品和試劑之間的反應后樣品顏色將有變化,然后用比色法測量氨氮【NH3-N】的量,使用推薦HI97715、HI96715、HI833XX系列等光度測定儀測定顏色變化,氨氮【NH3-N】的濃度以mg / L(ppm)顯示讀數。
溫馨提示:HI97715?HI833XX系列具有通過【▼▲】鍵轉換,將氨氮【NH3-N】讀數轉換成氨【NH3】讀數或者銨【NH4+】讀數

 

適用型號:意大利哈納HI93715、HI96715中量程氨氮測定

測量范圍:0.00 to 9.99 mg/L NH3-N

類型:水劑

預測次數:100次

環氧活化瓊脂糖凝膠6B Epoxy activated Sepharose 6B使用說明書

環氧活化瓊脂糖凝膠6B Epoxy activated Sepharose 6B使用說明書

環氧活化瓊脂糖凝膠6B
品牌: 金畔生物
英文名稱: Epoxy activated Sepharose 6B
說明:分離試劑
產品簡介
環氧活化瓊脂糖凝膠6B以6%瓊脂糖凝膠為基質,采用環氧活化的方法激活瓊脂糖上的羥基,活化后即可直接用于各種配基的偶聯??捎糜诟鞣N含有氨基、巰基或羥基的親和配基(蛋白質、多肽、氨基酸或糖等)的偶聯。具有使用方便、偶聯條件溫和、偶聯效率高和應用范圍廣的特點。
GRADE: BR
基質: 6%高度交聯瓊脂糖凝膠
功能基團: epoxy
配基密度: 20-40umoles/mL drained matrix
平均粒徑: 90um
最大流速: 300cm/h
偶聯條件: pH8-10,溫度 4 45°C時間1-16小時,可在水相和有機相中偶聯
用途:親和層析介質
性狀:白色膠體;
儲存溫度: 4-8°C
使用說明:
1、溶液的準備
偶聯液:0.1M Na2CO3, pH8. 5-10.0
封閉液: 1M乙醇胺, pH8.0
清洗液1: 0.1M 乙酸~乙酸鈉,0.5M NaCI, pH4.0
清洗液2: 0.1M Tris-HCl, 0.5M NaCI, pH8.0
保護液:含20%乙醇的1XPBS
2、樣品準備
樣品用偶聯液溶解,濃度約5-10mg/ml。
3、樣品偶聯
1)取適量的環氧活化瓊脂糖凝膠6B,去除保護液,離子水清洗三次,用偶聯液清
洗一次。
2)將溶解好的樣品中溶解后轉入清洗好的環氧活化瓊脂糖凝膠6B中填料:樣品.
溶液體積比約為1:1。
3) 25- 40°C搖床中振蕩混合反應24h。注:確保樹脂懸浮起來,否則會大大影響偶聯效率。
4)反應完后收集偶聯樣品,以便檢測偶聯效率。偶聯液清洗一遍。
5)加入等體積的封閉液,37 度振蕩混合反應1h。
6)將上述反應體系取出,流干其中的溶液,用3倍柱體積的去離子水清洗樹脂,清洗液1、去離子水、清洗液2和去離子水
重復沖洗2次,然后保存在等體積的保護液中,于29C-89C保存。
注意事項:
1.不同配基的偶聯方法與上面類似,但是反應的條件例如:配基加入量,反應時間,反應溫度應根據配基的不同進行適當的
調整
2.可用碳酸鹽、硼酸鹽和磷酸鹽緩沖液做為偶聯緩沖液,但絕對不能使用含有氨基的Tris、甘氨酸的緩沖液。

下面簡要介紹環氧活化瓊脂糖微球偶聯含氨基配基的使用過程

環氧活化瓊脂糖微球FF 是將環氧基團鍵合在瓊脂糖微球 6FF 上凍干形成的一種活性中間體,可直接偶聯含氨基、巰基和羥基的配基制備分離介質,用于生物大分子的純化分離。

  • 干粉的溶脹(非干粉形態忽略此步驟)

稱取一定量的環氧活化高流速瓊脂糖微球凍干粉,在蒸餾水中懸浮溶脹(1g 干粉約溶脹為 3~4ml?凝膠),放置大約 1?小時后抽濾,并用約 200ml(PH?7)去離子水洗滌干凈其中的添加劑,并在漏斗中抽干。

  • 配基的偶聯

一般配基偶聯的過程如下:

  • 偶聯溶劑:可用去離子水、碳酸鹽緩沖液和磷酸鹽緩沖液。不能用含氨基和其他含親核試劑的溶液。有時需要有機溶劑溶解配基,如用 50%二甲基甲酰胺和二氧六環等。
  • 取一定量的配基溶解到偶聯用的緩沖溶液中,使其完全溶解,最低濃度為200μmol

配基/ml?凝膠。凝膠與緩沖溶液以?1:0.5?到?1:1?的比例混合懸浮反應。

  • 將溶脹的凝膠懸浮到含有配基的緩沖溶液中,在20℃~45℃的水浴中搖蕩?16?小時。也可以用其他攪拌方法使配基偶聯,請勿用磁力攪拌。
  • 反應完全后,再洗滌掉緩沖液中的多余的配基。
  • 殘余活性基團的消除,將反應完洗滌過的凝膠懸浮到 1mol/L乙醇胺(PH?8)的溶液中,在 40℃~50℃最少反應 4 小時或者室溫反應過夜。
  • 在反應完后,用含有 5mol/LNaCl 的 0.1mol/L 醋酸緩沖液(PH 4.0)和含有 0.5mol/LNaCl 的偶聯緩沖液(PH 8.3)交替洗滌,至少重復以上循環洗滌 3 次。
  • 裝柱

⑴讓所有的材料和試劑達到室溫。配制初始緩沖液(平衡液)和洗脫緩沖液。

⑵根據柱子大小取所需量的凝膠,清洗掉溶液,抽干,用初始緩沖液(按凝膠:緩沖液

=3:1??的比例)配成勻漿。勻漿作脫氣處理。

⑶將柱內及柱子底端用水或緩沖液潤濕并保持一小段液位(液面略高于濾膜),務必使底端無氣泡。

⑷用玻璃棒引導勻漿沿著柱內壁一次性倒入柱內,注意勿使產生氣泡。打開柱子出液口,使凝膠在柱內自由沉降,連結好柱子頂端柱頭。

⑸打開蠕動泵,讓緩沖液用使用時流速的 1.33?倍的流速流過,使柱床穩定。用 2~3?倍柱體積的緩沖液平衡柱子。

  • 平衡

讓平衡緩沖液以一定流速流過柱子,到流出液電導和?pH?不變。平衡緩沖液一般是根據需要純化的蛋白來決定用哪種緩沖液。

  • 上樣

⑴樣品用平衡液配制,渾濁的樣品要離心和過濾后上樣。

⑵一般情況是讓目標產品結合在柱子上,用平衡液洗去雜質,再選擇一種洗脫液洗下目標產品。

⑶介質對樣品組分吸附的程度取決于介質配基的親和作用、樣品的親和性質、流動相的組成和溫度。

(4)再用緩沖液平衡到流出液電導和?pH?不變。

  • 洗脫

洗脫液也要根據需要純化的蛋白決定。

  • 再生

對于使用之后的凝膠,以 2~3?倍柱體積的 0.1mol/L?Tris·HCl+0.5mol/L?NaC(l?PH=8.5)

和?0.1mol/L?NaAc+0.5mol/L?NaCl(PH=4.5)依次反復沖洗?3~4?次,進行再生。

  • 在位清洗

根據偶聯的配基選擇在位清洗的方法,以洗去柱子污染物同時不傷害柱子為原則。對于一般的污染,用?0.1mol/L?NaOH?進行較長時間的清洗,對配基基本沒有的影響;當嚴重污染后,可用?0.5mol/L?NaOH??進行短時間的清洗;對于介質中含有其他試劑的,可用含有?8?mol/L尿素和?6?mol/L??的鹽酸胍的溶液進行在位清洗。若有失活蛋白或脂類物質洗不干凈,可用非離子表面活性劑如?0.1%?Triton?洗滌。

清洗完畢后,用至少?3?倍緩沖液平衡柱子。

  • 注意

在裝柱、使用和保存柱子的時候,要避免柱子流干,氣泡進入。

Stemgent mRNA 重編程試劑盒 Stemgent mRNA Reprogramming Kit

Stemgent mRNA 重編程試劑盒 Stemgent mRNA Reprogramming Kit

Stemgent mRNA 重編程試劑盒

Stemgent?mRNA Reprogramming Kit

◆產品概況

Stemgent ?mRNA 重編程試劑盒與?microRNA Booster 試劑盒(00-0073)配套使用可以迅速,安全,有效制備人 iPS 細胞。該方法不發生整合,不含病毒,與臨床研究相關。與慢病毒,仙臺病毒和其他方法相比,16天內制備 iPS 細胞,3周內細胞可進行傳代培養。慢病毒,仙臺病毒和其他方法還需要花20周時間確認沒有病毒殘留。該系統完全免除了病毒相關的生物污染問題的顧慮。

Stemgent??mRNA 重編程試劑盒包括5種重編程因子,監測轉染效率的細胞核 GFP 標記蛋白,Stemgent Pluriton™ 重編程培養基, B18R 蛋白。試劑盒和操作方法都經過充分的檢測和驗證。該試劑盒已經能夠將人 BJ 成纖維細胞進行重編程,該細胞可作為對照。Stemgent??mRNA 重編程試劑盒可以使研究正更容易調控蛋白表達水平的時間,量化控制重編程因子。

◆優勢

● 非整合-無需進行基因組整合

● 快速重編程,至少 12 天制備克隆

● 量化控制各種重編程因子

● 不需要進行多次傳代和細胞克隆篩選

● 在正常細胞和疾病細胞上進行驗證

● 提供培訓課程(收費)和在線支持

blob.png blob.png
?使用 mRNA 重編程系統制備 iPS 時程圖片。在第 6-10 天觀察到的

iPS細胞形態(放大 10×)。

?使用 mRNA 重編程系統制備 iPS 時程圖片,第 12 天和第 14 天。

第14天用?TRA-1-60 熒光染色(10×)。

◆相關產品

貨號 名稱 規格
00-0073 microRNA?Booster?Kit

microRNA增強試劑盒

1kit
00-0071 Stemgent??mRNA?Reprogramming?Kit

mRNA?重編程試劑盒

1kit
00-0075 Stemgent??StemRN™?SR?Reprogramming?Kit

StemRNATM?-SR重編程試劑盒

1kit
產品編號 產品名稱 產品規格 產品等級
00-0071 Stemgent??mRNA?Reprogramming?Kit
Stemgent??mRNA?重編程試劑盒
1kit

注:本品已停產,升級版本已推出:Stemgent??StemRNA™ 3rd Generation Reprogramming Kit(又名 StemRNA-NM Reprogramming Kit,產品編號:00-0076)。

StemRNA 3rd Gen Reprogramming Kit? StemRNA第三代重編程試劑盒

StemRNA 3rd Gen Reprogramming Kit

StemRNA第三代重編程試劑盒?(又名StemRNA™-NM重編程試劑盒)

Stemgent RNA重編程的新產品——StemRNA第三代重編程試劑盒(舊名StemRNA-NM重編程試劑盒),結合了未經修飾的RNA和microRNA技術,使干細胞研究人員追求的多功能性、簡易性和時間效益提升到一個新的層次,使人成纖維細胞,以及來自難以重編程的患者血液和尿液的細胞樣品得已重編程,成為具有全能性的誘導型多功能干細胞(iPS)。

◆主要優點

● 運用靈活技術,可從多種靶細胞得到高質量的人iPS細胞系

● 即開即用??捎糜趤碜云つw(成纖維細胞),血液(內皮祖細胞; EPCs)和尿液(尿源性上皮細胞; UDCs)細胞的重編程。

● 高效率的非整合重編程

● StemRNA-3rd Gen僅需要少至四種額外的試劑。試劑盒中的雙鏈microRNA可增強重編程效率,提高iPS獲得效率(成纖維細胞高達4%,EPC高達3%,UPC高達0.5%)。

● 省時的操作更快獲得結果,從而提高吞吐量

● 從成纖維細胞的細胞系中得到iPS克隆需要10-14天,而從EPC或UPC中獲得細胞系需要12-16天。無需篩選,無需像其他技術般額外培養2-10周(3-10代)以清除載體。

StemRNA 3rd Gen Reprogramming Kit (00-0076)
特點 成纖維細胞 尿液 (UDCs) 血液(EPCs)
每個試劑盒可轉染的孔數(6孔板) 9 3 3
轉染次數 4 6-8 6-8
獲得原代iPS細胞克隆所需天數 10-14 12-14 12-14
重編程效率 2-+4% 0.1-0.5% 0.4-3%
篩選
兼容異源成分的培養步驟
GMP級的RNA制備步驟

 

2914803910301306.jpg

在NutriStem™ XF / FF培養基(01-0005)中擴增后,使用StainAlive™ TRA-1-60抗體(09-0068)和DAPI對UDCs的P7原代iPS細胞進行染色。

圖片.png

StemRNA 3rd Gen Reprogramming Kit

2914803910415135.jpg

以StemRNA 3rd Gen Reprogramming Kit 配合NutriStem™ XF/FF培養基(01-0005)及iMatrix-511(NP892-011)基質上培養成纖維細胞衍生的iPS細胞7代。 放大倍數:4x

291567043798.png

StemRNA-NM的成纖維細胞重編程s時間表

?◆產品說明

規格 ?1 kit
試劑盒組分 ?OKSMNL NM-RNA(編號05-0040),30 μg,1 vial
EKB NM-RNA(編號05-0041),22 μg,1 vial
NM-microRNAs(編號05-0042),15 μg,1 vial
儲存與穩定性 ?1. 試劑盒三種成分均需儲存在-70°C以下;
2. 在適當的保存條件下,自到貨后三個月內有效。
質量控制 ?試劑盒種每種mRNA的大小與完整性均經過檢測。

StemRNA 3rd Gen Reprogramming Kit 成功進行了人成纖維細胞和人臍靜脈內皮細胞RNA基礎重編程功能驗證。

iPS細胞系完全重編程可通過多能性標記物的表達及正確的形態來確認。

試劑盒所有組分均無菌,支原體檢測均呈陰性。

使用許可 ?該產品使用Biontech AG獨家許可技術,引自專利WO 2009/088134。

Biontech AG的WO 2007/036366,WO 2007/036366,WO 2007/036366和WO 2007/036366等多專利也涵蓋

了本產品。

◆產品列表

產品編號 產品名稱 規格
00-0076 Stemgent??StemRNA™ 3rd Generation Reprogramming Kit
(StemRNA-NM Reprogramming Kit)
1 kit

◆相關產品

?產品編號 產品名稱 包裝
01-0005 NutriStem XF/FF Culture Medium (500 mL)
NutriStem 無飼養層干細胞培養基
500 mL
01-0005-100 NutriStem XF/FF Culture Medium (100 mL)

NutriStem 無飼養層干細胞培養基

100 mL
03-0002 Stemfactor™ FGF-Basic, Human Recombinant
Stemfactor™ FGF-Basic,人重組
50 μg
01-0020-50 NutriFreez D10 Cryopreservation Mediu 50 mL

iMatrix-511

產品編號 產品名稱 包裝
NP892-011 iMatrix-511
iMatrix-511層粘連蛋白
350 μg
NP892-012 1050 μg
NP892-013 175 μg
385-07361 iMatrix-511 solution(0.5 mg/mL)
重組層粘連蛋白511-E8片段,溶液(0.5 mg/mL)
175 μg×2

 

產品編號 產品名稱 產品規格 產品等級
00-0076 StemRNA™-NM?Reprogramming?Kit
StemgentRNA™-NM重編程試劑盒
1?kit

芬蘭百得BIOHIT移液器

芬蘭百得BIOHIT移液器

百得(BIOHIT)是一家跨國生物科技公司,開發,生產和市場,如移液器和吸頭以及診斷測試和分析系統的液體處理產品。 我們的產品用于研究,衛生保健和全球實驗室。 百得液體處理業務現在屬于賽多利斯實驗室產品與服務的業務區域。

百得簡介

●由Suovaniemi教授成立于1988年

●業務范圍:液體處理和診斷

●總部設在芬蘭赫爾辛基。

●附屬公司位于法國,德國,英國,俄羅斯,印度,中國,日本和美國。 在新加坡有一個代表處。

●生產工廠位于芬蘭和中國。

●百得的產品銷往約70個國家的450多個分銷商。

創新和專利

百得不斷地致力于創新和發展。 百得以其新的技術,產品和服務及研究成果用于開發的早期發現和預防疾病的安全和符合成本效益的實驗室工作以及診斷測試的液體處理解決方案。Z446通用型中容量高速離心機產品包括用于研究機構,醫療保健和工業實驗室和手動、電動移液器,吸頭并提供使用技巧以及維修和校準服務。

百得全球集團總部設在芬蘭赫爾辛基,在法國,德國,英國,俄羅斯,印度,中國,日本和美國的分公司,并在新加坡設有代表處。 此外,百得的產品銷往約70個國家的450個分銷商。

百得產品

手動移液器

電動移液器

移液控制器

瓶口分液器

連續分液器

移液器支架

移液工作站

移液器吸頭及耗材類

百得Proline?手動移液器

經濟可靠的選擇

百得家族最初的手動移液器系列?Proline?至今仍在各實驗室被使用,證明了其歷久彌新、實用可靠的特性。作為?Sartorius?手動移液器中最經濟型的一個系列,Proline?適合高等院校及尋求經濟實惠的液體處理工具的各種實驗室。由于其重量較輕且具有較高的準確度和精確度,也同樣受到專業人士的喜愛。

性能規格及訂購信息

產品編號 通道數 量程?(μl) 可變量 測量體積 系統誤差 隨機誤差 安全圓錐過濾器 Optifit SafetySpace™
(μl) (μl) (%) (%) 標準型 增強型 吸頭?(μl) 濾芯吸頭?(μl)
720010 1 0.1-2.5 μl 0.05 2.5 2.50 2.00 10 10
1.25 3.00 3.00
0.25 12.00 6.00
720015 1 0.5-10 μl 0.10 10 1.00 0.80 10 10
5 1.50 1.50 200 20
1 2.50 1.50
720080 1 2-20 μl 0.50 20 0.90 0.40 721008 721018 200 20
10 1.20 1.00 120
2 3.00 2.00
720025 1 5-50 μl 0.50 50 0.60 0.30 721008 721018 200 120
25 0.90 0.60 350
5 2.00 2.00
720050 1 10-100 μl 1.00 100 0.80 0.20 721007 721017 200 120
50 1.00 0.40 350 200
10 3.00 1.00 300
720070 1 20-200 μl 1.00 200 0.60 0.20 721007 721017 200 200
100 0.80 0.30 350 300
20 2.50 0.80
720320 12 5-50 μl 0.50 50 1.00 0.50 721014 200 120
25 1.50 1.00 350 200
5 3.00 2.00 300
720340 12 50-300 μl 5.00 300 0.70 0.25 721014 350 300
150 1.00 0.50
50 1.50 0.80

特性規格

產品編號 通道數 量程
(μl)
可變量
(μl)
測量體積
(μl)
系統誤差
(%)
隨機誤差
(%)
725010 1 0.1 – 3 0.002 3?/?1.5 / 0.3 1.3 / 2.40 / 10.00 0.80 / 1.60 / 6.00
725020 1 0.5 – 10 0.01 10 /?5 / 1 1.00 / 1.50 / 2.50 0.60 / 1.00 / 1.50
725030 1 2 – 20 0.02 20 / 10 / 2 0.90 / 1.20 / 3.00 0.40 / 1.00 / 2.00
725050 1 10 – 100 0.10 100 / 50 / 10 0.80 / 1.00 / 2.00 0.15 / 0.40 / 1.00
725060 1 20 – 200 0.20 200 / 100 / 20 0.60 / 0.80 / 2.30 0.15 / 0.30 / 0.90
725070 1 100 – 1000 1.00 1000 / 500 / 100 0.70 / 0.70 / 2.00 0.20 / 0.20 / 0.50
725080 1 500 – 5000 10.0 5000 / 2500 / 500 0.50 / 0.60 / 2.00 0.20 / 0.30 / 0.60
725090 1 1 – 10 ml 20.0 10000 / 5000 / 1000 0.60 / 1.20 / 3.00 0.20 / 0.30 / 0.60
725120 8 0.5 – 10 0.01 10 / 5 / 1 1.50 / 2.50 / 4.00 1.00 / 2.50 / 4.00
725130 8 5 – 100 0.10 100 / 50 / 10 0.70 / 1.00 / 3.00 0.25 / 0.70 / 1.50
725140 8 30 – 300 0.20 300 / 150 / 30 0.60 / 1.00 / 2.00 0.25 / 0.50 / 1.00
725220 12 0.5 – 10 0.01 10 / 5 / 1 1.50 / 2.50 / 4.00 1.00 / 2.50 / 4.00
725230 12 5 – 100 0.10 100 / 50 / 10 0.70 / 1.00 / 3.00 0.25 / 0.70 / 1.50
725240 12 30 – 300 0.20 300 / 150 / 30 0.60 / 1.00 / 2.00 0.25 / 0.50 / 1.00

DEAE-纖維素 DE-52 使用說明

DEAE-纖維素 DE-52 使用說明
DEAE-纖維素(52)
使用說明書
簡介
DEAE-纖維素,它采用平均粒徑為50μm的顆粒型親水高分子聚合物,表面又用大
分子糖鏈接枝,使它有更高的比表面積和更好的生物兼容性,它在高流水下保持
更高載量,同時又具有更好的分辨率。由于比表面積大,平衡和洗脫的時間也更.
短。它經過接枝即使是純化病毒,質粒等超大分子的物質,載量基本保持不變。

關于DEAE52纖維素的處理只有簡單的幾步,如下:

1.先將干粉的纖維素浸泡在蒸餾水中,一段時間大約3小時左右,我偏愛這個時間,去除雜質,最好抽干一下;
2.再用0.5mol/l的HCl溶液浸泡2小時,用去離子水洗凈至PH中性,并抽干;
3.將抽干的纖維素再浸泡在0.5mol/l的NaOH溶液中2小時,用去離子水洗至中性,抽干。即可用了
對于用過的纖維素,可以重復利用多次,但需要經過再生處理后才可以使用。再生處理可先用高濃度NaCl (1-2 mol/L)過柱沖洗柱床,以除去DEAE-52陰離子交換纖維所吸附的成份。然后,再用0.5 mol/L的HCl和NaOH處理,處理方法與預處理完全相同。

DEAE-纖維素的處理及裝柱
(1)處理
本實驗采用的是DEAE-纖維素DE 52 是弱酸型陰離子交換劑,具體處理方法為:先將DE52陰離子交換劑干粉浸泡于蒸餾水中,去除雜質;再在0.5N的HCL溶液中浸泡1-2h,再用無離子水或蒸餾水洗至pH值中性或PH4以上,并將其在抽濾漏斗中抽干;將抽干的離子交換劑浸泡在0.5N的NaOH溶液中1-2h,再用無離子水或蒸餾水將其洗至中性。
(2)裝柱
將層析柱清洗干凈垂直固定到層析架上,加1/3體積的無離子水,打開下出液口,水流暢通,即刻將小燒杯中裝有適宜濃度的柱材,輕輕倒入層析柱中,凝膠自然慢慢沉降再層析柱底部,凝膠沉積直到離層析柱上端1.5-2cm處,停止裝柱。層析柱上端進液口連接恒流泵,下出口連接蛋白質監測儀,待層析柱的平衡。
(3)平衡

在柱層析上樣前必須對層析柱進行平衡,所謂平衡就是將層析柱中的溶液用層析過程的緩沖液(洗脫液)置換出來,使層析柱中的緩沖系統與柱層析過程中的系統一致。其方法是:利用層析柱上端的恒流泵將平衡緩沖液泵入到層析柱內,打開層析柱下端的出口,平衡液流速在0.5-1ml/min,當下出口流出液的PH值與平衡緩沖液的PH值一致時,層析柱達到了平衡。在本實驗中,用0.002M Tris-HCL緩沖液PH7.4(內含0.0001M?EDTA),預先將DE-52柱進行平衡。

DEAE—纖維素的活化

稱取IgDEAE32或52,放入5ml量筒中,加蒸餾水浸泡過夜,觀察溶脹后DEAE的體積。根據所需層析柱的柱床體積計算所需DEAE的用量,稱取所需DEAE用蒸餾水浸泡過夜,其間換幾次水,每次除去細小顆粒。抽干,改用0.5ml/LNaOH溶液浸泡1h以上,抽干(可用布氏漏斗),用無離子水漂洗,使pH至8左右(用pH試紙檢查)。再改用0.5ml/LHCl溶液浸泡1h以上,去酸溶液,用無離子水洗至pH6左右。本實驗中在用前應以0.0175mol/L,pH6.7磷酸鹽緩沖液,浸泡平衡后使用。

DEAE—纖維素的再生
用過的離子交換劑可以反復使用,使其恢復原狀的方法俗稱“再生”。再生并非每次用酸、堿反復處理,通常只要“轉型”處理即可。所謂轉型就是使交換劑帶上所希望的某種離子,如希望陽離子交換劑帶上NH+4則可用NH4OH浸泡,如希望陰離子交換劑帶上Cl—則用NaCl溶液處理。本實驗中DEAE—纖維素在酸堿處理后,用0.0175mol/L,pH6.7磷酸鹽緩沖溶液浸泡即可轉型,以HPO4取代DEAE中的OH—。一般由于DEAE—纖維素使用后因帶有大量雜蛋白,所以再生時,先用0.5ml/L NaOH浸洗,用去離子水洗至pH8左右,再轉型,即可再使用。

三、應用的注意事項:
1)色譜柱裝填
1、所需要用到的材料的溫度要與色譜操作的溫度一樣,液體最好做脫氣處理。
填料可直接稱量需要的量用緩沖液溶漲一小時裝柱即可.
2、在柱子下端加入20%乙醇,以除去柱子中的空氣,關閉柱子出口,在柱
內保留少量的20%乙醇。20%乙醇容易產生氣泡,可以在里面加1%吐溫避免氣泡產生。也可以換成純水裝柱子,但是需要把填料中的20%乙醇也換成純水,具體
的方法取需要體積的填料在抽濾漏斗上進行,也可以小心傾去填料上的20%乙
醇,再換成5倍體積的純水,反復沉淀去上清,5次左右就可以用于裝柱子。
3、此填料顆粒比較細,所以一定 要注意柱子要選擇合適的篩網,不能漏,
也可以取點填料加到篩網.上試試,如果沒有問題再將填料連續倒入柱子時,要用
玻璃棒的緊靠柱子內壁引流,以減少氣泡的產生,讓填料先自然沉降到填料體積
不再變化,而填料和上面的液體很好分層,上層溶液完全澄清,就可以開泵用適
當的流速壓柱子,填料體積不再變化后,再把轉換頭緊頂在填料上就可以平衡柱
子使用。使用的流速要小于裝柱子的流速。
4、在裝柱子前,填料從冰箱中取出至少要室溫放置2-3個小時,這樣避免
裝柱子時由于溫度變化而使柱子中產生氣泡。
2)蛋白的結合
樣品的鹽濃度和pH要盡量和平衡柱子的緩沖液-致,鹽濃度過高或者pH帶
低也許掛不上,所以要根據自己的樣品做適當調整。
3)蛋白的洗脫
這個填料如果采用線性梯度洗脫,柱子的直徑和高度比最好是大于10,數值
越大越有利于分離,而且樣品最好別上太多,可以按約10mg/ml.上樣,如果采用
階段洗脫的方法,裝短粗柱子就可以,上樣量也沒有限制。階段洗脫容易放大,
重復性好,如果洗脫條件好完全可以得到和線性梯度一樣或者更好。采用什么方
法完全根據自己需要。
四、再生清洗
1、每次用完最好用含2M NaCl洗5個床體積,再用水洗5個柱床體積,然后用
20%乙醇保存,使用3-5次后在水洗之后再用70%的乙醇或30%異丙醇都含1%吐
溫洗5個柱床體積,最后20%的乙醇流洗5個柱床體積。
2、有機溶劑和水混合很容易產生氣泡,為了避免這樣情況,可以把配好的有
機溶劑在室溫放置過夜,再使用,這樣可以避免氣泡進柱子而導致柱子不能正常
使用。
五、保存
在20%乙醇中,4’C下長期保存。

63410-01-多聚賴氨酸包被載玻片

63410-01-多聚賴氨酸包被載玻片

1.??多聚賴氨酸包被載玻片poly-L-lysine?coated?slides

包被載玻片所用的多聚賴氨酸的分子量在150000-300000之間。首先配制0.1%的多聚賴氨酸水溶液,貯存于-20℃。包被時,取出溶液由載玻片的一段滴上,使其慢慢印跡,直至完全覆蓋玻片。多聚L賴氨酸包被的膜一定要確保很薄,經過風干即可使用。在一般免疫組化實驗中,多聚L賴氨酸不影響實驗的結果。但是在免疫膠體金-銀染的實驗中,一定要謹慎,可能會引起沉淀反應。

貨號 產品描述 規格
63410-01 Poly-L-Lysine?Coated?Slides 72/bx
63410-02 Poly-L-Lysine?Coated?Slides 144/bx

圓形蓋玻片,德國玻璃(Round Cover Slips, German Glass)

1.用于神經細胞培養?2.?用于難貼壁細胞培養(會顯出很好的貼壁效果)

3. #1的厚度大約0.13-0.16mm

4.?每包裝重28克

5. 6孔細胞培養板,以及12孔、24孔細胞培養板,均有適宜規格的圓形蓋玻片。

6.?適用于細胞培養板的圓形玻璃片,由美國公司精心打造,滿足您實驗中的一切需求!

品牌 貨號 直徑 厚度 片數
Electron Microscopy Sciences 72196-12 12?mm #1?(0.13?to?0.16mm) 650片/箱
Electron Microscopy Sciences 72196-15 15?mm #1?(0.13?to?0.16mm) 378片/箱
Electron Microscopy Sciences 72196-25 25?mm #1?(0.13?to?0.16mm) 160片/箱

Plastic and Glass Microscope Slides

Including Plastic, Glass, Quartz, Permanox™, Polystyrene, PTFE Coated, Poly-L-Lysine, and Silane Treated

Millennia 2.0 Adhesion Slidesarrow13Millennia™ 2.0 Adhesion Slides, 75 x 25 mm

Millennia™ 2.0 Slides have a higher density of binding site, which significantly increases the adhesive forces between the slide and the anchored tissue section; Millennia™ 2.0 Slides are ideal for immunohistochemistry, can tolerate harsh chemicals with no undesirable background staining, tissue artifact, or specimen loss.

71863-01 Millennia 2.0 Adhesion Slide, White 144/pk 59.00 Add to Cart
71863-02 Millennia 2.0 Adhesion Slide, Aqua 144/pk 59.00 Add to Cart
71863-03 Millennia 2.0 Adhesion Slide, Blue 144/pk 59.00 Add to Cart
71863-04 Millennia 2.0 Adhesion Slide, Burnt Orange 144/pk 59.00 Add to Cart
71863-05 Millennia 2.0 Adhesion Slide, Green 144/pk 59.00 Add to Cart
71863-06 Millennia 2.0 Adhesion Slide, Lilac 144/pk 59.00 Add to Cart
71863-07 Millennia 2.0 Adhesion Slide, Pink 144/pk 59.00 Add to Cart
71863-08 Millennia 2.0 Adhesion Slide, Tan 144/pk 59.00 Add to Cart
71863-09 Millennia 2.0 Adhesion Slide, Yellow 144/pk 59.00 Add to Cart

Millennia 2.0 Adhesion Slidesarrow13Diamond Etch™ Adhesion Slides, 90°

Slides which offer optimized etched labeling resolution. White glass, designed with top/bottom contrasting colors, specifically for slide etching instruments. Exhibits higher visibility with contrasting background.

71865-01 Diamond Etch™ Adhesion Slide, White 144/pk 56.00 Add to Cart
71865-02 Diamond Etch™ Adhesion Slide, Blue 144/pk 56.00 Add to Cart
71865-03 Diamond Etch™ Adhesion Slide, Green 144/pk 56.00 Add to Cart
71865-04 Diamond Etch™ Adhesion Slide, Lilac 144/pk 56.00 Add to Cart
71865-05 Diamond Etch™ Adhesion Slide, Pink 144/pk 56.00 Add to Cart
71865-06 Diamond Etch™ Adhesion Slide, Yellow 144/pk 56.00 Add to Cart
71865-07 Diamond Etch™ Non-Adhesion Slide, White 144/pk 38.00 Add to Cart
71865-08 Diamond Etch™ Non-Adhesion Slide, Blue 144/pk 38.00 Add to Cart
71865-11 Diamond Etch™ Non-Adhesion Slide, Pink 144/pk 38.00 Add to Cart
71865-12 Diamond Etch™ Non-Adhesion Slide, Yellow 144/pk 38.00 Add to Cart

arrow13InkPro™ Slides

White glass, specifically designed and optimized for use with ink jet slide printing instruments. Print quality exhibits high resolution bar codes and alphanumerical characters for efficient and error-free scanning.

71866-01 InkPro™ Slide, White 144/pk 23.00 Add to Cart
71866-02 InkPro™ Slide, Aqua 144/pk 23.00 Add to Cart
71866-03 InkPro™ Slide, Blue 144/pk 23.00 Add to Cart
71866-04 InkPro™ Slide, Green 144/pk 23.00 Add to Cart
71866-05 InkPro™ Slide, Lilac 144/pk 23.00 Add to Cart
71866-06 InkPro™ Slide, Pink 144/pk 23.00 Add to Cart
71866-07 InkPro™ Slide, Yellow 144/pk 23.00 Add to Cart

Positive Charge Microsope Slidesarrow13Positive Charge Microscope Slides

These new slides have a positive charge that makes the surface ideal for cell, tissue and general sample adhesion. Typical samples have a negative charge and are therefore attracted to the positively charged slide surface, significantly reducing sample “float”.

Slides are made of high clarity glass, 25 x 75 mm and are 1.1 mm thick (compatible with most printers). Frosted marking surface on one end. Packed 72 pieces per plastic box/1440 per case (20 packs).

71873-01 45° corners positive charged, frosted end 72/pk 20.00 Add to Cart
71873-02 90° corners positive charged, frosted end 72/pk 20.00 Add to Cart

Control Slides Helicobacter Humanarrow13Control Slides Helicobacter Human

Tissue Control Slides; one slide in every pack has a serial section stained using the helicobacter human staining procedure.

71871 Control Slides Helicobacter Human 25/pk 125.00 Add to Cart

HiQa™ Microscope Slidesarrow10HiQa™ Microscope Slides

HiQa™ Microscope Slides are high quality assured. They have 90 degree ground edges for safety and are available as Plain or Twinfrosted, and in Mega and SupaMega sizes.

These high quality slides are cellophane wrapped, pre-cleaned, ready to use, and suitable for use with automatic coverslipping machines.

71881-30 HiQa™ Supa Mega Twinfrost Slide, 1.0-1.2 mm. Sq ground edge 100/pk 43.00 Add to Cart
71881-40 HiQa™ Supa Mega Plain Slide, 1.0-1.2 mm. Sq ground edge 100/pk 39.00 Add to Cart
71881-50 HiQa™ Mega Twinfrost Slide, 1.0-1.2 mm. Sq ground edge 100/pk 37.00 Add to Cart

Histobond? + Supa Mega Slidesarrow10Histobond? + Supa Mega Slides

Histobond? + Supa Mega Slides are designed for mounting large sections of neuro and breast tissue. Manufactured from the highest quality white glass, with an innovative positive charge coating, Histobond? + Supa Mega slides offer exceptional adhesive properties and provide a superior coverage of reagents across the slide.

These slides have a ‘Right Side’ indicator (RS) to help ensure users correctly orientate the slide, as well as a 7 mm marking area across the width of the slide in order to maximize the working area.

71881-60 Histobond? + Supa Mega Slides 75.2 x 50.4 1 mm, White 50/pk 79.00 Add to Cart

HiQa™ Mega and Supa Mega Cover Slipsarrow10HiQa™ Supa Mega Cover Slips

HiQa™ Supa Mega Cover Slips are made from transparent hydrolytic class 1, flaw-free glass.

They are guaranteed to be of the highest quality.

71881-90 HiQa™ Supa Mega Cover Slips No. 1.0, 50 x 64 mm 100/pk 33.00 Add to Cart

Marienfeld Superior Uncoated Soda Lime Glass Microscope Slidesarrow10Marienfeld Superior™ Uncoated Soda Lime Glass Microscope Slides

EMS introduces the Marienfeld Superior Uncoated Soda Lime Glass Microscope Slides. They are used for the preparation and archiving of specimens, as well as for microscopic investigation.

These slides measure 76 mm x 26 mm x 1 mm thick. They are wettable, made from soda lime glass, pre-cleaned, and ready for use.

71872 Soda Lime Glass Slides 50/pk 12.00 Add to Cart

Microscope Glass Slides - Escoarrow13Microscope Glass Slides

Our microscope glass slides are manufactured with reliability and quality to be the best.

  • Made from the highest quality glass sheets
  • The dimensions of the slides have been standardized to millimeters according to the U.S. government Specifications
  • They are packed with care to assure cleanliness
  • The glass is fully annealed to minimize chipping

Our microscope glass slides are precleaned and are made from clear-white glass. They are individually inspected and free from moisture, dirt, and film. All of our slides are flat, uniform, durable, and cellophane wrapped.

Chemical Makeup

Green (%) White (%)
Silicon Dioxide 72.2 72.15
Sodium Oxide 14.5 14.25
Calcium Oxide 6.5 6.25
Magnesium Oxide 4.4 4.1
Aluminum Oxide 1.5 1.12
Potassium Oxide 0.3 1.15
Sulfur Trioxide 0.3 0.3
Iron Oxide 0.1 0.3
Titanium Oxide 0.05 0.05

Physical Properties

Glass Density 2.4024 g/cm3
Softening Point 724°C
Annealing Point 545°C
Strain Point 511°C
Dispersion Value (Nu Value) 64
Dimensional Variance ±1.5000
Thermal Expansion (0-300°C) 8.36 x 106/ °C
Dielectric Constant @ 20°C (68°F) (1 MHz):ET = 6.7
Young’s Modulus E = 10,000,000 lbs/sq in.
Torsion Modulus G = 4,000,000 lbs/sq in.
Poisson’s Ratio μ = 0.2
Luminous Transmittance @ 0.040: 91.8%

arrow13Microscope Slide, Plain End

Catalog # Thickness Size, mm Pack Price
71880-01 1.2 mm 25 x 75 Gross 38.50 Add to Cart
71880-10 1.2 mm 25 x 75 10 Gross 370.00 Add to Cart
71880-20 1.2 mm 25 x 75 20 Gross 699.00 Add to Cart

arrow13Microscope Slide, Frosted End

These slides have 3/4″ frosting on one end which allows for easy marking.

Catalog # Thickness Size,mm Pack Price
71870-01 1.2 mm 25 x 75 Gross 44.00 Add to Cart
71870-10 1.2 mm 25 x 75 10 Gross 430.00 Add to Cart
71870-20 1.2 mm 25 x 75 20 Gross 750.00 Add to Cart

arrow10Microscope Slide, Sandblasted, Frosted End

These slides have a 3/4″ sandblasted, frosted end.

25 x 75 mm x 1 mm (T)

Catalog # Thickness Size,mm Pack Price
71877-19 1 mm 25 x 75 Gross 43.00 Add to Cart
71877-20 1 mm 25 x 75 10 Gross 398.00 Add to Cart

Microscope Slide, SUPERFROST? Endarrow13Microscope Slide, SUPERFROST? End

With a superfrosted end. These slides have one side which is frosted with a brilliant white opaque coating that will take and retain all pen markings. They offer superior readability and they are permanent.

Catalog # Thickness Size,mm Pack Price
71867-01 1 mm 25 x 75 Gross 37.75 Add to Cart
71867-10 1 mm 25 x 75 10 Gross 342.00 Add to Cart
71868-01 1.2 mm 25 x 75 Gross 47.00 Add to Cart
71868-10 1.2 mm 25 x 75 10 Gross 420.00 Add to Cart

Microscope Slide, SUPERFROST? PLUS - Adhesion Slidearrow13SUPERFROST? PLUS – Adhesion Slide

These Superfrost Plus(+) glass slides are made by a new process which offers the following features

  • They place a permanent positive charge in the microscope slide
  • They electrostatically attract frozen tissue sections and cytology preparations, binding them to the slide
  • They form a bridge so that covalent bonds develop between formalin fixed sections and the glass
  • Tissue sections and cytological preparations adhere better to the Plus glass slides without the need for special adhesives or protein coatings

Benefits

  • Plus glass puts an end to background staining
  • It eliminates blue or red background staining which is often encountered in hematoxylin and eosin staining of tissue sections mounted on albuminized glass
  • No brown background staining occurs when immuno-peroxidase or?in situ?DNA procedures are performed on our Plus glass. A higher degree of enzyme digestion can be performed on plus glass without the loss of tissue sections
  • Cytological preparations placed on Plus glass resist cell loss during enzyme digestion, DNA denaturation and RNA hybridization
  • Plus glass improves tissue adherence in microwave staining
  • Chromic acid washes are redundant for molecular hybridization because the slides are RNAse-free
  • Plus slides are Color coded to help keep track of specimens during processing as well as filing

The slides measure: 25 x 75 mm, 1 mm thick.

71869-10 Superfrost Plus Slide, White 1 Gross 83.00 Add to Cart
71869-11 Superfrost Plus Slide, White 10 Gross 795.00 Add to Cart
71869-20 Superfrost Plus Slide, Blue 1 Gross 85.00 Add to Cart
71869-21 Superfrost Plus Slide, Blue 10 Gross 830.00 Add to Cart
71869-30 Superfrost Plus Slide, Green 1 Gross 85.00 Add to Cart
71869-31 Superfrost Plus Slide, Green 10 Gross 830.00 Add to Cart
71869-40 Superfrost Plus Slide, Pink 1 Gross 85.00 Add to Cart
71869-41 Superfrost Plus Slide, Pink 10 Gross 830.00 Add to Cart
71869-50 Superfrost Plus Slide, Yellow 1 Gross 85.00 Add to Cart
71869-51 Superfrost Plus Slide, Yellow 10 Gross 830.00 Add to Cart

technical dataSuperfrost? Plus Gold Slidesarrow13SUPERFROST? PLUS Gold Slides

These slides are specially designed for the application of fresh or formalin fixed frozen tissue. They are inherently tissue binding so there is no need to add sprays or adhesives to the slides.

Features

  • Securely attach frozen breast, brain, skin and other normally difficult to bond tissue sections to the slide
  • Ideal when special stains, immunocytochemical and in situ DNA hybridization techniques are applied on fresh and fixed frozen tissue
  • Assist tissue adherence during immunocytochemical analysis of estrogen and progesterone receptors in fresh frozen breast tissue
  • They are compatible with both toluidine blue and hematoxylin and eosin rapid frozen section stains
71864-01 Superfrost Plus Gold Slides 25/bx 38.00 Add to Cart
71864-10 Superfrost Plus Gold Slides 10bx/cs (250) 360.00 Add to Cart

Colormark™ Plus Glass Slidearrow13Colormark™ Plus Glass Slide

Specimen Slide identification is VITAL. Automated etched slide labeling instruments ensure specimen slide integrity but are difficult to read on ordinary slides. To clearly read diamond scribed slide labels against any surface, you need the only slide designed to be used with your automated slide labeling instrument. You need COLORMARK slides.These brightly printed slides greatly improve etched labeling visibility and offer all the advantages of Superfrost¨ slides. A bright contrasting color has been printed on the back of the Superfrost end so that patient or specimen information etched through the painted end on the front is clearly visible. Colormark Plus slides are adhesive coated therefore ensuring the adhesion of the section to the slide during staining procedures.

63400-10 White Frosted End Front, Black frosted End Back 1 gross 82.00 Add to Cart
63400-11 White Frosted End Front, Black frosted End Back 10 gross 775.00 Add to Cart
63400-20 Blue Frosted End Front, Blue Frosted End Back 1 gross 83.00 Add to Cart
63400-21 Blue Frosted End Front, Blue Frosted End Back 10 gross 778.00 Add to Cart
63400-30 Green Frosted End Front, Green Frosted End Back 1 gross 84.00 Add to Cart
63400-31 Green Frosted End Front, Green Frosted End Back 10 gross 798.00 Add to Cart
63400-40 Pink Frosted End Front, Red Frosted End Back 1 gross 85.00 Add to Cart
63400-41 Pink Frosted End Front, Red Frosted End Back 10 gross 805.00 Add to Cart
63400-50 Lavender Frosted End Front, Red Frosted End Back 1 gross 84.00 Add to Cart
63400-51 Lavender Frosted End Front, Red Frosted End Back 10 gross 798.00 Add to Cart
63400-60 Lavender Frosted End Front, Black Frosted End Back 1 gross 84.00 Add to Cart
63400-61 Lavender Frosted End Front, Black Frosted End Back 10 gross 798.00 Add to Cart

arrow13Control Slides

Use our Histology Control Slides and you will always know for sure which specimen is the Patients and which is the Control.

Features

  • Patient and Control tissue samples on the same slide
  • Positive identification of control and patient samples
  • Proof of correct staining procedures
  • Utmost adhesion of samples to slide throughout staining procedure
  • Easy and permanent identification of slides

Available in two configurations: Style #329+ with ? inch of frosted area; style 334+ with 1 inch frosted area.

63448-10 Control Slide 329+ 144/pk 78.00 Add to Cart
63448-20 Control Slide 334+ 144/pk 82.00 Add to Cart

Cytology Slidearrow13Special Glass Slides

arrow13Cytology Slide: 1 x 3″ (25 x 75 mm), 1 mm Thick

Superfrost letters VCE and lines on the same side as the Superfrost end.

71874-01 Cyto-Slide Letters VCE & Lines 1 gross 42.00 Add to Cart
71874-10 Cyto-Slide Letters VCE & Lines 10 gross 395.00 Add to Cart

Superfrost letters VC and lines on the same side as the Superfrost end.

71875-01 Cyto-Slide Letters VC & Lines 1 gross 42.00 Add to Cart
71875-10 Cyto-Slide Letters VC & Lines 10 gross 395.00 Add to Cart

71876-01arrow13Full-Frosted Cytology Microscope Slides

Full frosted cytology microscope slides. 75 mm (L) x 25 mm (W), 1 mm thick.

  • Surface frosting over one entire side of slide.
  • Frosting disappears when coverslip is mounted.
  • Does not interfere with viewing.
71876-01 Full-Frosted Cytology Slide 144/pk 48.00 Add to Cart
71876-10 Full-Frosted Cytology Slide 10/pk/case 430.00 Add to Cart

arrow13Round Corner Microslides

71887-152 Round Corner Microslides 144/pk 38.00 Add to Cart

Fluorescent Antibody Slides with Etched Ringsarrow13Slides with Etched Rings

Useful for Fluorescent Antibody Examination. The size of the slide is 3″ x 1″, approximately 1 mm thick, with an etched frosted end. Pre-cleaned. Packed 72/box.

71879-10 Two Etched 10 mm Circle Slide Gross 40.00 Add to Cart
71879-11 Two Etched 10 mm Circle Slide 10 Gross 385.00 Add to Cart
71879-15 Two Etched 15 mm Circle Slide Gross 40.25 Add to Cart
71879-16 Two Etched 15 mm Circle Slide 10 Gross 392.00 Add to Cart

uperFrost™ Clipped Corner Slidearrow13SuperFrost™ Clipped Corner Slide

Designed specially for the Sysmex SP-100 slide prep unit and recommended for the Diagnostics. The 45 degree clipped corner slide offers exceptional cleanliness and resistance to sticking. The tight tolerances for the length and width help to reduce accumulation of glass chips within the instrument. The pre-cleaned slide is 25 x 75 mm and approximately 1 mm thick. Ideal for technicians, who routinely prepare large number of slides.

71882-01 SuperFrost™ Clipped Corner Slide 1 gross 45.00 Add to Cart
71882-10 SuperFrost™ Clipped Corner Slide 10 gross 430.00 Add to Cart

Large Glass Slidesarrow13Large Glass Slides

In addition to our comprehensive collection of glass slides manufactured by ESCO we now carry large glass slides which are available in 75 x 38 mm and 75 x 51 mm. These slides are precleaned, individually selected and are suitable for large specimen sections, such as, brain tissue.

71860-01 75 x 38 mm Glass Slide, 1 mm Thick Gross 58.00 Add to Cart
71862-01 75 x 51 mm Glass Slide, 1.2 mm Thick Gross 80.00 Add to Cart

arrow13Glass Slides

Our square microscope glass slides are made from clear, white, pre-cleaned glass. They are moisture, dirt, and film free. All of our slides are flat, consistent, and stand up to repetitive use. They are made from the highest quality glass sheets. The slides’ dimensions meet U.S. Government standards. They are carefully packed to assure cleanliness and the glass is fully annealed to minimize chipping. Glass thickness is 1 mm.

71881-10 Glass Slide, 25 x 25 mm, Ground Edges 36/pk 72.00 Add to Cart
71881-20 Glass Slide, 76 x 100 mm, Plain Cut Edges 36/pk 81.00 Add to Cart

arrow13ClariTex Slides and Coverslips IFU

These ready-to-use slides are manufactured from super grade glass with uniform thickness, fulfilling optical requirements for light microscopy.

71887-201arrow13ClariTex Plain Slides

Edges are ground with 90° corners. Available in packs of 1000.

  • Plain slide for routine use.
  • Pre-cleaned and ready to use.
  • Dimensions: 26.0 (W) x (76.0 (D) mm.
71887-201 ClariTex Microscope Slides, Plain 1000/pk 60.00 Add to Cart

71887-202arrow13ClariTex Singlefrost Slides

These slides have a 20 mm frosted end on one side. Edges are ground with 90° corners. Available in packs of 1000.

  • Frosted section at one end.
  • Pre-cleaned and ready for use.
  • Dimensions: 26.0 (W) x 76.0 (D) mm.
71887-202 ClariTex Singlefrost Microscope Slides 1000/pk 48.00 Add to Cart

71887-203arrow13ClariTex Twinfrost Slides

These slides have a 20 mm frosted end on both sides. Edges are ground with 90° corners. Available in packs of 1000.

  • Frosted section on both sides.
  • Pre-cleaned and ready for use.
  • Dimensions: 26.0 (W) x 76.0 (D) mm.
71887-203 ClariTex Twinfrost Microscope Slides 1000/pk 58.00 Add to Cart

arrow13ClariTex ColourCoat Slides

These slides are recommended for labeling or marking with a pencil or pen. Their 20 mm frosted area is resistant to all routine chemicals and stains used in laboratories. WhiteCoat slides are available with 45° clipped corners.

71887-20471887-20671887-20771887-20871887-20971887-21071887-211

  • 20 mm silky color frosted area.
  • Available in six colors.
  • Pre-cleaned and ready to use.
  • Dimensions: 26.0 mm (w) x 76.0 mm (l).
  • Thickness: 1.1 ± 0.1 mm.
71887-204 ClariTex WhiteCoat Microscope Slides 1000/pk 77.00 Add to Cart
71887-205 ClariTex WhiteCoat Slides, Clipped Corner 1000/pk 80.00 Add to Cart
71887-206 ClariTex GreenCoat Microscope Slides 1000/pk 77.00 Add to Cart
71887-207 ClariTex BlueCoat Microscope Slides 1000/pk 77.00 Add to Cart
71887-208 ClariTex OrangeCoat Microscope Slides 1000/pk 77.00 Add to Cart
71887-209 ClariTex PinkCoat Microscope Slides 1000/pk 77.00 Add to Cart
71887-210 ClariTex YellowCoat Microscope Slides 1000/pk 77.00 Add to Cart
71887-211 ClariTex PurpleCoat Microscope Slides 1000/pk 77.00 Add to Cart

71887-212arrow13ClariTex Supa Mega Plain Slides

These double-width slides allow sections to be much larger. Pathologists can spend less time switching between slides that hold smaller sections. Edges are ground with 90° corners. Available in packs of 100.

  • Pre-cleaned and ready to use.
  • Dimensions: 52.0 (W) x 76.0 (D).
71887-212 ClariTex Supa Mega Plain Microscope Slides 100/pk 39.00 Add to Cart

72887-213arrow13ClariTex Supa Mega Twinfrost Slides

These double-width slides have a 10 mm frosted end on both sides. They can be marked with a pencil or pen. Edges are ground with 90° corners. Available in packs of 100.

  • Pre-cleaned and ready to use.
  • Dimensions: 52.0 (W) x 76.0 (D) mm.
71887-213 ClariTex Supa Mega Twinfrost Microscope Slides 100/pk 40.00 Add to Cart

arrow13ClariTex Coverslips

ClariTex Coverslips are for in vitro diagnostic applications. They ensure that specimens are secure and protected. Their flat, smooth surface will maintain optical quality even when archived for several years.

  • Manufactured from green soda-lime glass.
  • Uniform size and flatness.
  • Sealed in aluminum tropical packs.

ClariTex Coverslips No. 1.0 Thickness

71887-214 24 x 50 mm 1000/pk 58.00 Add to Cart
71887-215 22 x 50 mm 1000/pk 54.00 Add to Cart
71887-216 22 x 22 mm 1000/pk 21.00 Add to Cart
71887-217 Supa Mega, 50 x 64 mm 100/pk 33.00 Add to Cart

ClariTex Coverslips No. 1.5 Thickness

71887-218 24 x 50 mm 1000/pk 40.00 Add to Cart
71887-219 22 x 50 mm 1000/pk 21.00 Add to Cart
71887-220 22 x 22 mm 1000/pk 20.00 Add to Cart
71887-221 Supa Mega, 50 x 64 mm 100/pk 30.00 Add to Cart

Microscope Slide – Gold Sealarrow13Microscope Slide – Gold Seal?

We proudly introduce GOLD SEAL? microscope slides for your convenience.

GOLDSEAL? slides are made from pure white glass (sodalime) and at accurately cut sizes. To preserve the highest quality from manufacturer to ultimate user, all GOLD SEAL? slides are hermetically sealed in our Moisture-Barrier™ system, providing a dry-slide environments during shipment and storage.

UltraStick™ (adhesion coating: APTES, 3-Aminopropyl Triethoxysilane) – Adhesion coated micro slides- one of the best from GOLD SEAL? are ideal for histological and cytological applications demanding superior specimen-to-slide adhesion. The enhanced silane coating eliminates tedious procedures and special additives to your water bath.

Procedures such as immunoperoxidase, in situ DNA hybridization, enzyme digestion and frozen tissue processing will benefit from the amplified adhesion characteristics of this product. Overall cost savings and assured quality of your prepared specimen will be recognized with UltraStick™ from Gold Seal?.

arrow13Plain Slides
Catalog # Gold Seal Description Pack Price
63710-05 3011 3 x 1″, 1 mm Thick 1 gross 43.00 Add to Cart
63710-25 3011 3 x 1″, 1 mm Thick 25 gross 830.00 Add to Cart
63711-05 3048 3 x 1″, 1.2 mm Thick 1 gross 45.00 Add to Cart
63711-25 3048 3 x 1″, 1.2 mm Thick 25 gross 860.00 Add to Cart
63712-05 3010-P 3 x 1″, 1 mm Thick – Cytologentics processing 1 gross 43.00 Add to Cart
63712-25 3010-P 3 x 1″, 1 mm Thick – Cytologentics processing 25 gross 850.00 Add to Cart
arrow13Patented Moisture-Barrier™ enclosing 5 gross inner packages
Catalog # Gold Seal Description Pack Price
63718-05 3054 25 x 75 mm, 1 mm Thick, Plain 5 gross 236.00 Add to Cart
63718-25 3054 25 x 75 mm, 1 mm Thick, Plain 25 gross 875.00 Add to Cart
63719-05 3055 25 x 75 mm, 1 mm Thick, White Rite-On™ Frost 5 gross 310.00 Add to Cart
63719-25 3055 25 x 75 mm, 1 mm Thick, White Rite-On™ Frost 25 gross 1,215.00 Add to Cart
arrow13Rite-On™ Frosted Slides with brilliant white epoxy ink
Catalog # Gold Seal Description Pack Price
63721-05 3050 25 x 75 mm, 1 mm Thick, Frosted One End, One Side 1 gross 55.00 Add to Cart
63721-25 *** 25 x 75 mm, 1 mm Thick, Frosted One End, One Side 25 gross 1,075.00 Add to Cart
63722-05 3050-P 25 x 75 mm, 1 mm Thick, Frosted End, One Side, Cytogentics Processing 1 gross 55.00 Add to Cart
63722-25 3050-P 25 x 75 mm, 1 mm Thick, Frosted End, One Side, Cytogentics Processing 25 gross 1,075.00 Add to Cart
63723-05 3049 3 x 1″, 1.2 mm Thick, Frosted One End, One Side 1 gross 64.00 Add to Cart
63723-25 3049 3 x 1″, 1.2 mm Thick, Frosted One End, One Side 25 gross 1,350.00 Add to Cart

***Gold Seal equivalent product offered

arrow13UltraFrost™ Frosted Slides, white epoxy ink frosted one end, one side
Catalog # Gold Seal Description Pack Price
63728-05 3063 25 x 75 mm, 1 mm Thick, Frosted one End with Gold Seal Logo 1 gross 58.00 Add to Cart
63728-25 3063 25 x 75 mm, 1 mm Thick, Frosted one End with Gold Seal Logo 25 gross 1,090.00 Add to Cart
63729-05 3064 25 x 75 mm, 1 mm Thick, Frosted one End with Pre-Print Patient Information Label and Gold Seal? Logo 1 gross 60.00 Add to Cart
63729-25 3064 25 x 75 mm, 1 mm Thick, Frosted one End with Pre-Print Patient Information Label and Gold Seal? Logo 25 gross 1,245.00 Add to Cart
arrow13Fluorescent Antibody Slides
Catalog # Gold Seal Description Pack Price
63732-05 3032 3 x 1″, 1.2 mm Thick with Two 10 mm diameter circles, Frosted one Side, one End with White Epoxy Ink, including Circles 1 gross 84.00 Add to Cart
63732-02 3032 3 x 1″, 1.2 mm Thick with Two 10 mm diameter circles, Frosted one Side, one End with White Epoxy Ink, including Circles 25 gross 1,720.00 Add to Cart
arrow13UltraStick™/UltraFrost™ Adhesion Slides frosted one end, one side with epoxy ink
Catalog # Gold Seal Description Pack Price
63734-01 3039 25 x 75 mm, 1 mm Thick, Surface Coated with APTES for Adhesion 1 gross 73.00 Add to Cart
63734-10 3039 25 x 75 mm, 1 mm Thick, Surface Coated with APTES for Adhesion 10 gross 690.00 Add to Cart

technical dataQuartz Microscope Slides and Coverslipsarrow13Quartz Microscope Slides and Coverslips

There are many applications that require slides and coverslips that have absolute UV transparency, and high temperature properties. EMS now is introducing the highest quality, clear fused quartz microscope slides and coverslips to meet all of these requirements.

  • The window of highest transparency to UV is between 0.15μ to 5μ
  • Typical flatness measured 2 to 4″ bands per inch
  • Soften point is about 1250°C (2282°F)
  • Standard microscope slide thickness is 1 mm
  • Standard coverslip thickness is 0.2 mm
arrow13Quartz Microscope Slides
72250-01 Slide 1″ x 1″ (25 x 25 mm), 1 mm thick each 14.00 Add to Cart
72250-02 Slide 2″ x 1″ (50 x 25 mm), 1 mm thick each 21.50 Add to Cart
72250-03 Slide 3″ x 1″ (75 x 25 mm), 1 mm thick each 24.00 Add to Cart
72250-04 Slide 2″ x 3″ (50 x 76 mm), 1 mm thick each 75.00 Add to Cart
72250-05 Slide 10 x 10 mm, 1 mm thick each 16.00 Add to Cart

These slides have all of the same properties as the ones above; the only difference is these are 0.5 mm thick.

72250-06 Slide 1″ x 1″ (25 x 25 mm), 0.5 mm thick each 28.00 Add to Cart
72250-07 Slide 2″ x 1″ (50 x 25 mm), 0.5 mm thick each 32.00 Add to Cart
72250-08 Slide 3″ x 1″ (75 x 25 mm), 0.5 mm thick each 34.00 Add to Cart
arrow13Quartz Coverslips
72255-02 Coverslip Rd 1″ (25 mm), dia. each 29.00 Add to Cart
72256-02 Coverslip Sq 1″ x 1″ (25 x 25 mm) each 29.00 Add to Cart
72256-03 Coverslip Rd 18 mm (0.71″) x 0.2 mm Thick (#2) each 35.00 Add to Cart
72256-04 Coverslip Rd 25.4 mm (1″) x 0.2 mm Thick (#2) each 29.00 Add to Cart
72256-05 Coverslip Rd 33.02 mm (13.0″) x 0.2 mm thick (#2) each 39.00 Add to Cart
72256-06 Coverslip Rd 25 mm (0.984″) x 0.15-0.18 mm thick (#1) each 46.00 Add to Cart
72256-07 Coverslip Sq 25.4 mm (1″) x 0.2 mm thick (#2) thick each 46.00 Add to Cart
72256-08 Coverslip Sq 25.4 mm (1″) x 0.15-0.18 mm thick (#1) each 62.00 Add to Cart

Permanox™ and Polystyrene Microscope Slidesarrow13Permanox™ and Polystyrene Microscope Slides

Permanox™ is a strong, biologically inert material resistant to non-chlorinated hydrocarbons, acetone, and other EM reagents. Permanox™slides allow you to do cell fixation and staining on the slide; they can be cut with scissors. Permanox™ has not been shown to be autofluorescent. All of the microscope slides are 1″ x 3″.

NOTE: Long exposure to solvents will cause the slide to warp. Xylene and Toluene based mounting media should be avoided. Glycerol, gelatin, and other aqueous based media are recommended.

71885 Permanox™ Microscope Slide 20/pk 80.00 Add to Cart
71886 Permanox™ Microscope Slide 100/pk 338.00 Add to Cart
71888 Polystyrene Microscope Slide 20/pk 76.00 Add to Cart
71889 Polystyrene Microscope Slide 100/pk 311.00 Add to Cart

Disposable Rinzl Plastic Micro Slidesarrow11Disposable Rinzl Plastic Micro Slides

  • Made from optical clear vinyl with a refractive index of glass
  • Ideal for wet mount, urines, etc
  • Economical, they can be used and discarded
  • Available standard size 3 x 1″ and packaged 144 per box
  • Will not break, scratch, or cut fingers
  • Not auto-fluorescent
  • Thickness 0.0180 to 0.022″ or 0.46 to 0.56 mm
71890-12 Rinzl Plastic Micro Slides (A) 12/box 4.25 Add to Cart
71890-01 Rinzl Plastic Micro Slides (B) 144/box 13.00 Add to Cart
71890-10 Rinzl Plastic Micro Slides (B) 10 x 144/box 105.00 Add to Cart

Poly-L-Lysine Coated and Silane Treated Microscope Slidearrow13Poly-L-Lysine

Our premium glass slides with a frosted end are now available pre-coated with either Poly-L-Lysine or Silane (aminoalkysilane). These slides improve the adhesion of tissues to the slide.

63410-01 Poly-L-Lysine Coated Slides 72/bx 56.00 Add to Cart
63410-02 Poly-L-Lysine Coated Slides 144/bx 101.00 Add to Cart
63411-01 Silane Coated Slides 72/bx 56.00 Add to Cart
63411-02 Silane Coated Slides 144/bx 101.00 Add to Cart

Technical?Tip

Poly-L-Lysine Coating of Slides

Polysine™ Microscope Adhesion Slidearrow13Polysine™ Microscope Adhesion Slide

The permanent bio-adhesive material on our Polysine™ slide attracts frozen and paraffin embedded sections, centrifuge preparation and cytology smears.

63412-01 Polysine™ Adhesion Slide 72/bx 60.00 Add to Cart
63412-02 Polysine™ Adhesion Slide 2 x 72/bx 105.00 Add to Cart

Excell™ Adhesion Slidesarrow13Excell™ Adhesion Slides

The Excell slide was developed with superior adhesion and wettability properties, and has been shown to perform very well in most applications

  • Excell™ Adhesion Surface for Heat Induced Epitope Retrieval – Excell™ was developed for use in the HIER methods that require high pH antigen retrieval solutions, including EDTA. Formalin fixed paraffin embedded tissue demonstrated more consistent attachment when performing these procedures on the Excell™ surface
  • Automated Staining – Excell™ has a uniform hydrophilic surface that works extremely well with automated staining equipment
  • Cell Culture – Excell™ surface technology conditions the surface to enhance the attachment of anchorage dependent cell lines, including many fastidious lines, such as primary neuronal cells. Attachment and proliferation is increased so cells achieve a confluent monolayer sooner
  • Plastic – Excell™ works well for both Epon and Methyl Methacrylate sections. Its uniformly hydrophilic surface allows the sections to spread well during initial attachment. Section adherence is maintained throughout lengthy staining procedure
  • Laser Capture Microdissection – Excell™ gives you the advantage of excellent tissue adherence of all tissue types, yet it easily releases those area of interest identified for Laser Capture Microdissection.
64370-01 Excell™ Adhesion Slide 144/pk 112.00 Add to Cart

arrow13Adhesion Slide – TOMO?

These TOMO hydrophilic adhesion slides offer superior tissue adhesion, staining performance and slide surface fluidics. Proprietary slide coating ensures consistent performance even after exposure to heat stress and humid environmental conditions. Optimized for use with thermal printers.

These slides are recommended for use on Ventana? Benchmark staining platform and automated IHC platforms.

These slides are exceptionally flat, with low fluorescence glass, and finished with 90° and clipped corners.

The slides are packaged 100 per box, 10 boxes per case (1000 slides) and they measure 3″ x 1″ (75 x 25 mm).

Catalog# Description Qty. Price
63705-01 TOMO Adhesion Slide, White, 90° 100/pk 42.00 Add to Cart
63705-02 TOMO Adhesion Slide, White, 90° 1000/cs 390.00 Add to Cart
63705-03 TOMO Adhesion Slide, White, 45° 100/pk 42.00 Add to Cart
63705-04 TOMO Adhesion Slide, White, 45° 1000/cs 390.00 Add to Cart
63705-05 TOMO Adhesion Slide, Blue, 45° 100/pk 42.00 Add to Cart
63705-06 TOMO Adhesion Slide, Blue, 45° 1000/cs 390.00 Add to Cart
63705-07 TOMO Adhesion Slide, Green, 45° 100/pk 42.00 Add to Cart
63705-08 TOMO Adhesion Slide, Green, 45° 1000/cs 390.00 Add to Cart
63705-09 TOMO Adhesion Slide, Pink, 45° 100/pk 42.00 Add to Cart
63705-10 TOMO Adhesion Slide, Pink, 45° 1000/cs 390.00 Add to Cart
63705-11 TOMO Adhesion Slide, Yellow, 45° 100/pk 42.00 Add to Cart
63705-12 TOMO Adhesion Slide, Yellow, 45° 1000/cs 390.00 Add to Cart

Text Box:arrow13Adhesion Slide – TruBond™

Adhesive slides with proven performance under high temperature and pH conditions

These adhesive slides, including the hydrophilic TruBond™ 360, work well with automatic IHC applications. EMS is confident that the quality of these slides will exceed your expectations. For problems with tissue loss try our TruBond™ 380.

Available colors: White, Blue, Green and Yellow.

 

TruBond™ 380 TruBond™ 360
MAS-GP Adhesion Slides
TruBond™ 200
APS Adhesion Slide
Glass Type High Clarity, Low
Fluorescence White
High Clarity, Low Fluorescence White High Clarity, Low Fluorescence White
Surface Wettability Hydrophilic Hydrophilic Hydrophobic
Adhesion Very Strong Strong Strong
Edge Treatment Ground Corner Ground Corner Ground Corner
For Manual IHC Yes Yes Yes
For Automatic IHC Yes Yes Yes
For Fluorescence Yes Yes Yes
Recommended Applications – HIER processes when tissue type require extra strong adhesion
– Epitope enhancement & DNA probe procedures
– Autopsy & brain sectioning
– All IHC applications
– HIER pH 6 & pH 9 protocols
– Routine histology
– Frozen section
– All IHC applications
– Routine histology

Ordering

TruBond™ is packaged 100 slides per box, 10 boxes to a case.

Item# Description Color 100 slides/bx 10 boxes/cs
63700-Wx TruBond™ 380 White 40.00 Add to Cart 352.00 Add to Cart
63700-Bx TruBond™ 380 Blue 40.00 Add to Cart 352.00 Add to Cart
63700-Gx TruBond™ 380 Green 40.00 Add to Cart 352.00 Add to Cart
63700-Yx TruBond™ 380 Yellow 40.00 Add to Cart 352.00 Add to Cart
63701-Wx TruBond™ 360 White 40.00 Add to Cart 352.00 Add to Cart
63701-Bx TruBond™ 360 Blue 40.00 Add to Cart 352.00 Add to Cart
63701-Gx TruBond™ 360 Green 40.00 Add to Cart 352.00 Add to Cart
63701-Yx TruBond™ 360 Yellow 40.00 Add to Cart 352.00 Add to Cart
63702-Wx TruBond™ 200 White 40.00 Add to Cart 352.00 Add to Cart
63702-Bx TruBond™ 200 Blue 40.00 Add to Cart 352.00 Add to Cart
63702-Gx TruBond™ 200 Green 40.00 Add to Cart 352.00 Add to Cart
63702-Yx TruBond™ 200 Yellow 40.00 Add to Cart 352.00 Add to Cart
63703-Bx TruView™ Fine Blue 20.00 Add to Cart 104.00 Add to Cart
63703-Gx TruView™ Fine Green 20.00 Add to Cart 104.00 Add to Cart
63703-Yx TruView™ Fine Yellow 20.00 Add to Cart 104.00 Add to Cart

For Trubond Coverslips – click?here

arrow13LiveSlide? Living Slides

LiveSlide
LiveSlide

These innovative, optically clear microscope slides have proven to unmask rarely seen features of microorganisms.

A shallow reservoir confines them to a liquid depth not much larger than the depth of field of the microscope, yet deep enough to allow the microscopic animals to move about freely.

View more?videos

LiveSlide? Features

  • Uniform depth of large viewing well is 0.009″
  • Large viewing area has partitions to keep specimens in viewing area
  • Optically clear
  • Made of Makrolon? 2558 polycarbonate
  • Includes two reusable coverslips made of Makrofol?, 0.005″ and 0.010″
  • Excellent impact resistance for safety indoors and outdoors
  • Both the base and the coverslips are reusable
  • Can be used as a traditional slide simply by flipping the base over
  • Can be used with traditional and digital microscopes
  • 100% made in the USA
71877-01 LiveSlide each 3.75 Add to Cart
71877-05 LiveSlides 5/pk 17.50 Add to Cart
71877-10 LiveSlides 10/pk 34.00 Add to Cart
71877-50 LiveSlides 50/pk 160.00 Add to Cart

BrightSlide Darrow12BrightSlide™ D

Fluorescence Enhancing Slides and Versatile, (bio)-functionalized coatings for Glass, Metal Oxides and ITO

Fluorescence systems (e.g. microscopes) are today widely used in the life science research. For many applications an increased sensitivity compared to existing glass slides is required. The BrightSlide™ D technology uses a proprietary dielectric coating to significantly enhance the signal intensity of fluorescence molecules from the surface. It can be read out with all systems that illuminate and detect from the top. The technology makes use of purely optical principles and substitutes conventional glass substrates.

The BrightSlide™ D (Dielectric) comes standard as a bare SiO2?Surface.

BrightSlide D
Enhancement of the fluorescence on BrightSlide™ image of 150nm thick section of biological materialBrightSlide D
Fluorescence signal on standard, glass slide image of 150nm thick section of biological material

Benefits

  • Better signal to noise ratio
  • Less sample material can be used
  • More genes can be detected
  • Existing instrument platform can be used
  • Purely optical amplification of fluorescence signal
  • Dielectric, high quality SiO2?surface ready to apply customer bio-functional coating (use your own bio-functional coating, no change of chemical protocol required)

Applications

  • Universal detection platform for fluorescence detection on planar surfaces, e.g. fluorescence detection of ultra thin samples
  • Differential gene expression using Cy3 and Cy5 labels simultaneously
  • Gene expression using Cy3 or Cy5 labels independently

Specifications

Optimization 488 nm and 590 nm excitation wavelengths (other wavelengths on request)
Polarization Both s- and p-polarizations can be used for excitation and emission
Excitation Light 0° – 3° Angle of Incidence
Background Ultralow Fluorescence
Label Durable, chemically resistant with a unique serial number
Compatibility Suitable for most of commercially available fluorescence microscopes with illumination and detection from the top
Substrate Material D263T eco (other materials on request)
Substrate Format 75 ±0.05 mm x 25 ±0.05 mm x 1.1 ±0.1 mm (other formats on request)
Active Area slide: 63 mm x 22 mm
optical coating: 5/2 x 0.4; C2 x 0.4; E1.0 according to DIN ISO 10110
Chemical Composition (top surface) Clean, dielectric, SiO2?surface (ITO coating on request)
Environmental Stability (optical coating only) Stable against temperatures up to 200°C. All strongly oxidizing cleaning protocols can be used. All organic solvents can be used. Chemical reagents which etch SiO2(HF, NaOH, KOH) can destroy the enhancement effect of the BrightSlide™
Storage Store in clean environment at room temperature

Ordering Information

63426-D BrightSlide™ D each. 400.00 Add to Cart
63426-D-10 BrightSlide™ D 10/pk 3,900.00 Add to Cart
63426-D-100 BrightSlide™ D 100/pk 24,000.00 Add to Cart

Borosilicate Capillary Micro Glass Slidearrow12Borosilicate Capillary Micro Glass Slide

Made from Corning? 7740 borosilicate, low expansion, type I glass.

Micro glass slides are precision rectangular glass capillary tubes for use in microscopic viewing of liquid samples. Capillary action instantly collects the sample and the thin glass walls minimize evaporation. The design of these cells eliminates the needs for cover glass and the hanging drop method. These micro glass slides can withstand heat up to 500°C. Samples can be retained for future reference by sealing or cementing open ends. Micro glass slide are packaged in plastic vials.

I.D. tolerance ± 10%, Wall tolerance ± 20%

Catalog# Dimensions ID mm
Path x width
Wall
Thickness
Length mm Qty. Price
63820-05 0.02 x 0.2 0.020 50 30/vial 40.00 Add to Cart
63820-10 0.02 x 0.2 0.020 100 30/vial 64.00 Add to Cart
63821-05 0.03 x 0.3 0.025 50 33/vial 43.00 Add to Cart
63821-10 0.03 x 0.3 0.025 100 33/vial 75.00 Add to Cart
63822-05 0.04 x 0.4 0.030 50 36/vial 44.00 Add to Cart
63822-10 0.04 x 0.4 0.030 100 36/vial 78.00 Add to Cart
63823-05 0.05 x 0.5 0.040 50 44/vial 62.00 Add to Cart
63823-10 0.05 x 0.5 0.040 100 44/vial 108.00 Add to Cart
63824-05 0.10 x 1.0 0.090 50 40/vial 53.00 Add to Cart
63824-10 0.10 x 1.0 0.090 100 40/vial 92.00 Add to Cart
63825-05 0.20 x 2.0 0.150 50 36/vial 49.00 Add to Cart
63825-10 0.20 x 2.0 0.150 100 36/vial 88.00 Add to Cart
63826-05 0.30 x 3.0 0.300 50 31/vial 43.00 Add to Cart
63826-10 0.30 x 3.0 0.300 100 31/vial 71.00 Add to Cart
63827-05 0.40 x 4.0 0.400 50 28/vial 62.00 Add to Cart
63827-10 0.40 x 4.0 0.400 100 28/vial 96.00 Add to Cart
63828-10 0.50 x 5.0 0.300 100 14/vial 68.00 Add to Cart
63829-10 0.60 x 6.0 0.400 100 12/vial 57.00 Add to Cart
63830-10 0.70 x 7.0 0.460 100 9/vial 44.00 Add to Cart
63831-10 0.80 x 8.0 0.540 100 7/vial 59.00 Add to Cart
63832-10 0.90 x 9.0 0.600 100 6/vial 47.00 Add to Cart
63833-10 1.00 x 10.0 0.670 100 4/vial 56.00 Add to Cart
63840-05 0.05 x 1.0 0.030 50 50/vial 59.00 Add to Cart
63840-10 0.05 x 1.0 0.030 100 50/vial 114.00 Add to Cart
63841-05 0.10 x 2.0 0.100 50 45/vial 62.00 Add to Cart
63841-10 0.10 x 2.0 0.100 100 45/vial 102.00 Add to Cart
63842-05 0.20 x 4.0 0.150 50 36/vial 52.00 Add to Cart
63842-10 0.20 x 4.0 0.150 100 36/vial 85.00 Add to Cart
63843-05 0.30 x 6.0 0.300 50 24/vial 44.00 Add to Cart
63843-10 0.30 x 6.0 0.300 100 24/vial 79.00 Add to Cart
63844-05 0.40 x 8.0 0.400 50 18/vial 44.00 Add to Cart
63844-10 0.40 x 8.0 0.400 100 18/vial 79.00 Add to Cart

twin adhesive well stickers (1cm diameter) firmly adhere to microscope slidesarrow13Microscope Slide-Wells

Disposable Liquid-Holding Wells

Convenient?Pre-cut, twin adhesive well stickers (1 cm diameter) firmly adhere to microscope slides. Examine specimens with or without cover slips. Available in depths of 0.25 mm and 0.50 mm.

Multiple ApplicationsExamine or count tissue culture cells, yeast, bacteria and other microorganisms. Piggy-back with Microscope Slide-Grids to facilitate counting and sizing.

Versatile?– Compatible with different styles of microscopes (conventional, inverted or dissecting).

Chemically Inert?– Made of polyester with an acrylic adhesive. Microscope Slide-Wells are inert and stable under most laboratory conditions.

70366-16 Slide Wells, 0.25 mm thick 25/pk 44.00 Add to Cart
70366-13 Slide Wells, 0.50 mm thick 25/pk 56.00 Add to Cart

Disposable Adhesive Gridsarrow13Microscope Slide-Grids

Disposable Adhesive Grids

Convenient?– Easy-to adhere stickers having side-by-side twin grids (20 mm x 20 mm each with 1 mm line spacing) for microscope slides. Count or measure cells and estimate the size or length of microscopic or semi-micro objects

Versatile?– Compatible with different styles of microscopes (conventional, inverted or dissecting). Virtually all cells and specimens placed on top of Slide-Grids can be counted and/or sized.

Dry Or Wet?– Use with dry specimens or suspended specimens such as cells held in buffer under cover slips. Separate cover slips can be placed over each Slide-Grid.

Chemically Inert?– Made of polyester with an acrylic adhesive. Slide-Grids are inert and stable under most laboratory conditions.

70366-14 Slide Grids, 20 mm x 20 mm 40/pk 30.00 Add to Cart

Quick-Read Microscope Slides with Gridsarrow13Quick-Read™ Microscope Slide with Grid

These slides are especially manufactured to cater to the Microscopic Urinalysis testing. They are made from clear acrylic with 10 chambers, each chamber has 81 pre-measured wells that hold a determined volume of urinary sediment, giving fast and convenient microscopic examination of each specimen.

64633-01 Quick-Read Microscope Slide with Grids 100/pk 160.00 Add to Cart

Quick-Read Microscope Slidesarrow13Quick-Read™ Microscope Slide

These slides are especially manufactured to cater to the Microscopic Urinalysis testing. They are made from clear acrylic with 10 chambers, each chamber has 18 pre-measured circles that hold a determined volume of urinary sediment, giving convenient and easy microscopic examination of cellular elements in 1 ml of urine. The circles are able to be seen as a group at 100x magnification.

64633-02 Quick-Read Microscope Slide 100/pk 160.00 Add to Cart

Gridded Microscope Slidesarrow13Gridded Microscope Slides

These 3 x 1″ slides have an indexed grid on the reverse side of the frosted end. The ink is resistant to stains and common laboratory solvents. Each square grid fills one low-powered-field (approximately 100x magnification). These slides make it easy to locate an organism or cell every time. There are sixty-four squares in total. The slides are available with coatings of Poly-L-Lysine or they can be purchased plain (uncoated).

The grid measures 2 mm square-16 mm total. Numbers 1-8 and alpha A-H.

arrow131. Gridded Microscope Slides without hydrophobic coating.
63405-01 Grid Slides, Uncoated 72/pk 75.00 Add to Cart
63405-02 Grid Slides, Uncoated 144/pk 139.00 Add to Cart
63406-01 Grid Slides, Poly-L-Lysine 72/pk 82.00 Add to Cart
63406-02 Grid Slides, Poly-L-Lysine 144/pk 147.00 Add to Cart
arrow132. Gridded Microscope Slides with hydrophobic coating.
63408-01 Grid Slides, Round well 72/pk 98.00 Add to Cart
63409-02 Grid Slides, Square well 72/pk 98.00 Add to Cart

arrow13Cardboard “Well” Slides

EMS introduces these unique cardboard “well” slides. Complete with glass covered slide and aluminum holder, these slides are less prone to static as compared to similar plastic slides.

arrow131. Microscope slides made from cardboard housed in aluminum holders complete with glass coverslips.

Slides measure 76 mm (L) x 27.2 mm (W) x 4.5 mm (H)

Cardboard Slide
63515-01
Cardboard Slide
63515-02
Cardboard Slide
63515-03
Cardboard Slide
63515-04
Cardboard Slide
63515-05
Cardboard Slide
63515-06
Cardboard Slide
63515-07
Cardboard Slide
63515-08
Cardboard Slide
63515-09
Cardboard Slide
63515-10
Cardboard Slide
63515-11
Cardboard Slide
63515-12
arrow132. Double depth slides with paper top and acetate or glass cover slip.

Slides measure 74.7 mm (L) x 25.3 mm (W) x 3.6 mm (H)

Cardboard Slide
63515-13
Cardboard Slide
63515-14

Specifications

Cat. # Number of Wells Space/Well Dimensions Color
63515-01 1 Well, 64 Numbered Spaces 2.5 x 4.5 mm Black
63515-02 1 Well, 64 Numbered Spaces 2.5 x 4.5 mm White
63515-03 1 Well, 32 Numbered Spaces 4 x 4 mm Black
63515-04 1 Well, 32 Numbered Spaces 4 x 4 mm White
63515-05 10 Wells 5 mm dia. Black
63515-06 10 Wells 5 mm dia. White
63515-07 4 Wells 12 mm dia. Black
63515-08 4 Wells 12 mm dia. White
63515-09 2 Wells 15 mm dia. Black
63515-10 2 Wells 15 mm dia. White
63515-11 1 Well 15 mm dia. Black
63515-12 1 Well 15 mm dia. White
63515-13 1 Double Depth Well 15 mm dia. Black
63515-14 1 Double Depth Well 15 mm dia. White

Ordering Information

63515-01 Slide in Holder w/Glass Coverslip, 64 Spaces, Black 25/pk 90.00 Add to Cart
63515-02 Slide in Holder w/Glass Coverslip, 64 Spaces, White 25/pk 90.00 Add to Cart
63515-03 Slide in Holder w/Glass Coverslip, 32 Spaces, Black 25/pk 90.00 Add to Cart
63515-04 Slide in Holder w/Glass Coverslip, 32 Spaces, White 25/pk 90.00 Add to Cart
63515-05 Slide in Holder w/Glass Coverslip, 10 Wells, Black 25/pk 90.00 Add to Cart
63515-06 Slide in Holder w/Glass Coverslip, 10 Wells, White 25/pk 90.00 Add to Cart
63515-07 Slide in Holder w/Glass Coverslip, 4 Wells, Black 25/pk 90.00 Add to Cart
63515-08 Slide in Holder w/Glass Coverslip, 4 Wells, White 25/pk 90.00 Add to Cart
63515-09 Slide in Holder w/Glass Coverslip, 2 Wells, Black 25/pk 90.00 Add to Cart
63515-10 Slide in Holder w/Glass Coverslip, 2 Wells, White 25/pk 90.00 Add to Cart
63515-11 Slide in Holder w/Glass Coverslip, 1 Well, Black 25/pk 90.00 Add to Cart
63515-12 Slide in Holder w/Glass Coverslip, 1 Well, White 25/pk 90.00 Add to Cart
63515-13 Slide, Paper Top w/ Coverslip, 1 Double Depth Well, Black 25/pk 90.00 Add to Cart
63515-14 Slide, Paper Top w/ Coverslip, 1 Double Depth Well, White 25/pk 90.00 Add to Cart

PTFE Printed Slidesarrow13

PTFE Printed Slides

PTFE Printed Slides, are extremely hydrophobic, acetone proof, chemical resistant and autoclavable. They are perfect for controlling cross-contamination in staining, immunolabeling and immunofluorescence tests. All printed slides are precleaned. The well surface is 100% wettable, which enhances cell attachment and increases its spreading capabilities within the well. These slides minimize the use of expensive purified antigens and antibodies. The slides measure 3 x 1″ (76 x 26 x 1 mm).

EMS Printed Slides are manufactured to order offering maximum shelf life. The shelf life of PTFE Printed Slides range from 18 months to two years. However, it is recommended that all glass slides (Printed or un-printed) be used in a timely manner to avoid glass “weathering”, (slides becoming cloudy or hazy even with proper storage. All of our Printed Slides are autoclavable and have a frosted end. They are intended for one time use only.

Features

  • Super Hydrophobic Mask
  • Clean, Wettable Wells
  • A Bioadhesive Surface Treatment to Enhance Cell and Tissue Attachment
Catalog No. Number of Well Well Size Pack Price
63414-13 1 Ring 13 mm Dia. 72 69.00 Add to Cart
63414-10 Square 10.7 x 10.7 mm 72 62.00 Add to Cart
63415-12 1 Round 12 mm Dia. 72 89.00 Add to Cart
63415-32 1 Oval 24.4 x 16.7 mm 72 72.00 Add to Cart
63415-08 1 Round 8 mm Dia. 100/bx 96.00 Add to Cart
63416-08 2 Round 8 mm Dia. 72 96.00 Add to Cart
63416-15 2 Round 15 mm Dia. 72 82.00 Add to Cart
63417-13 2 Ring 13 mm Dia. 72 79.00 Add to Cart
63418-11 3 Ring 11.28 mm Dia. 72 79.00 Add to Cart
63419-08 3 Round 8 mm Dia. 72 97.00 Add to Cart
63419-12 3 Round 12 mm Dia. 72 79.00 Add to Cart
63419-14 3 Round 14 mm Dia. 72 79.00 Add to Cart
63419-VIR 3 Round TGH VIROLOGY 72 79.00 Add to Cart
63420-05 4 Round 5 mm Dia. 72 79.00 Add to Cart
63420-08 4 Round 8 mm Dia. 72 79.00 Add to Cart
63420-11 4 Ring 11.43 mm Dia. 72 79.00 Add to Cart
63421-07 5 Round 7 mm Dia. 72 79.00 Add to Cart
63421-10 6 Round 10 mm Dia. 72 79.00 Add to Cart
63421-11 Etched 5 Round 11.43 mm Dia. 72 79.00 Add to Cart
63423-05 6 Round 5 mm Dia 72 79.00 Add to Cart
63423-08 6 Round 8 mm Dia. 72 79.00 Add to Cart
63422-06 8 Round 6 mm Dia. 72 79.00 Add to Cart
63422-11 8 Round 11 mm Dia. 72 79.00 Add to Cart
63424-06 10 Round 6 mm Dia. 72 79.00 Add to Cart
63425-05 12 Round 5 mm Dia. 72 79.00 Add to Cart
63429-04 21 Round 4 mm Dia. 72 79.00 Add to Cart
63430-04 24 Round 4 mm Dia. 72 79.00 Add to Cart
63434-02 30 Round 2 mm Dia. 72 79.00 Add to Cart

PTFE Printed Slidesarrow13

PTFE Printed Slides

PTFE Printed Slides which are extremely hydrophobic and acetone proof, chemically resistant and autoclavable. The slides measure 3×1″ (76 x 26 x 1 mm), 72 slides per pack.

PTFE Printed Slides

Features

  • Super Hydrophobic Blue Mask
  • Clean Wettable Wells
Catalog Glass Size, Configuration Well/Ring
size mm
Price
63412-15 3″ x 1″, 2 ring, blue 15 90.00 Add to Cart

Macro/Latex Agglutinationarrow13

Macro/Latex Agglutination

25 to a pack

Catalog Glass Size, Configuration Well/Ring
size mm
Price
63450-31 4″ x 3″, 3 ring, yellow 26.4 198.00 Add to Cart
63450-32 4.25″ x 2″, 3 ring, white 26.4 96.00 Add to Cart
63453-61 4.4″ x 2.1″, 6, white oval 17.4 x 26 202.00 Add to Cart
63453-63 4.215″ x 2″, 6 white oval 17.4 x 26 202.00 Add to Cart
63456-15 4.331″ x 2.165″, 10 ring white 15 99.00 Add to Cart
63459-15 4.75″ x 3.5″, 15 ring white 15 202.00 Add to Cart
63460-15 3″x 2″ x 1.2 mm, 15 well 11.28 etched 202.00 Add to Cart

Lovins Micro-Slide Field Finderarrow13

Lovins Micro-Slide Field Finder

Marking a microscope slide to identify a particular field of interest for future reference has always been a difficult problem for many microscopists.

The Field Finder has a precision rectangular-coordinate grid pattern, which is accurately positioned on a special microscope slide. The slide has been carefully edge ground to provide straight edges and square corners to ensure repeatable results.

Lovins Micros-Slide Field Finder helps you to index points of interest on a microscope slide the same way you find them on a road map. A standard 75 x 25 mm glass slide has a photo etched grid pattern of 1 mm squares, subdivided into 0.1 mm intervals. Each square is marked with a letter and a number. You center the detail in the field of the microscope, replace the specimen slide with the field finder, and read the coordinates. Note reading on slide, and you can find that detail immediately by reversing procedure.

The pattern is imaged in vacuum deposited chrome for durability.

72266-01 Micro-Slide Field Finder, 75 x 25 mm each 95.00 Add to Cart

arrow13Retriever Tissue Slides

Retriever Tissue Slides are high adhesive glass microscope slides for paraffin-embedded tissue section, including tissue arrays. The slides were designed and optimized for the EMS Antigen Retriever (62700 series) to preserve the section attachment and tissue morphology during heat-induced epitope recovery in Retriever in pH 6 (Low), pH 9 (High), or Universal Retriever buffer.

Slides can also be successfully used in any other epitope-recovery technology (autoclave, microwave, proteolitic) and show superior performance over positively charged or polylysine-coated slides, especially in high pH buffers and recovery buffers containing EDTA.

These slides are ideal for both IHC and immunofluorescent applications, as well as for all antigen recovery systems.

Each of the slides have a custom imprinted frosted colored end, each color representing a different glass surface.

General Properties

  • Retriever Tissue Slides are made from soda lime glass
  • Dimensions approximately 76 x 26 mm, Thickness 1.0 mm (± 0.05 mm)
  • 90° ground edges
  • Colored or white marking area 20 mm, at one end, on one side
  • Pre-cleaned, ready for use
  • Autoclavable
  • Supplied in plastic boxes of 100 pieces
  • Certified for in-vitro-diagnostic (IVD) application

Specifications

71880-50 HistoBond? + F, Blue Both surfaces of these slides bond tissue sections adhesively with their positive charge. No additional adhesives are required. The reliable anchoring prevents tissue sections to float during washing and cooking. Reliable for routine IHC with FF/PE sections that involve antigen retrieval.
71880-50-CS HistoBond? + F, Blue
71890-50 HistoBond? + R, Orange Superior type of adhesion slide with a considerably higher positive charge than other regular charged slides. This results in strong adhesion of the tissue, even under alkaline conditions of EDTA/EGTA-containing buffers. IHC, IF, in-situ, hybridization, grade slides.
71890-50-CS HistoBond? + R, Orange
71890-51 HistoBond? + X, White Tissue sections anchor covalently on the glass surface. Even a non-polar tissue, e.g. very fatty (breast, brain, etc.) that does not cause a charge difference between the adhesive layer and the section, reliably bonds chemically to the glass.
71890-51-CS HistoBond? + X, White

Ordering

71880-50 Retriever Tissue Slides, HistoBond? + F, Blue 100/pk 90.00
71880-50-CS Retriever Tissue Slides, HistoBond? + F, Blue 20 pk/cs 1,350.00
71890-50 Retriever Tissue Slides, HistoBond? + R, Orange 100/pk 60.00
71890-50-CS Retriever Tissue Slides, HistoBond? + R, Orange 20 pk/cs 1,011.00
71890-51 Retriever Tissue Slides, HistoBond? + X, White 100/pk 64.00
71890-51-CS Retriever Tissue Slides, HistoBond? + X, White 20 pk/cs 1,081.00 Add to Cart

arrow13Petrographic Microscope Slides

Petrographic Slides

Glass slides made from fine white glass, with ground and polished edges. 72 slides to a box.

71534-01 Petrographic Microscope Slides, 46 x 27 x 1.2 mm 72/pk 60.00 Add to Cart

For Petrographic Slide Boxes, click?here.