Bio-serv F1258 液體酒精飼料 Lieber-DeCarli ’82, Ethanol說明書

貨號：F1258

英文名稱：Rodent Liquid Diet, Lieber-DeCarli ’82, Ethanol

規格：4 Liters/Bag

Directions for Preparing 4 Liters of Diet
These directions are for preparing diets that contain 36% kcal from ethanol. To prepare diets with modified
ethanol derived calories, see conversion chart.
1. Add dry mix, Product# F1258 (523.6 grams/4 Liters) to a blender container.
2. Measure 3400 mL of cold water (4 degrees Celsius) to the container.
3. Add 268 mL of 95% ethanol (not provided) to the container.
5. Blend for 20-30 seconds at low speed.
6. Product ready to dispense into the feeding tubes

準備 4 升飲食的說明
這些說明用于制備含有 36% 千卡乙醇的飲食。 準備含有改良的飲食
乙醇衍生的卡路里，見換算表。
1. 將干燥混合物 Product# F1258（523.6 克/4 升）添加到攪拌器容器中。
2. 稱量 3400 毫升冷水（4 攝氏度）到容器中。
3. 向容器中加入 268 mL 95% 乙醇（未提供）。
5. 低速攪拌 20-30 秒。
6. 準備分配到進料管中的產品

Feeding Instructions
It is recommended to feed the liquid diet in liquid diet feeding tubes as standard water sipper bottles can
become clogged with particulates. To fill the liquid diet feeding tubes, place thumb over the feed well and fill
with liquid diet from the top. Maintain thumb on the feed well while screwing cap on securely and invert tube
to release any trapped air bubbles. Place in feeding tube holder in cage.
The prepared diet should be maintained under refrigeration and should be dispensed within 3 days.
The prepared diet should not be kept longer than 48 hours at room temperature to prevent diet deterioration.
If diet has been prepared the previous day, invert bottle several times prior to dispensing to re-suspend
particles, which may have settled.
The average mouse will consume 20-30 mL of liquid diet per day. The average rat will consume 80-100 mL
of liquid diet per day. Intake will vary with the size and sex of the animal.
A gradual introduction of the liquid diet is recommended. Introduce the liquid diet in the presence of the
standard solid diet. Over a period of 3-5 days, increase the amount of liquid diet while decreasing the amount
of solid diet to allow the animal to acclimate. Additional acclimation time is required for ethanol containing
diets. It is recommended that the animals be gradually introduced to the ethanol diet as follows:
Day 1-3 Feed mixture of 1/3 ethanol diet and 2/3 control diet
Day 4-7 Feed mixture 2/3 of ethanol diet with 1/3 of control diet
Day 8 Feed ethanol diet only
The liquid diet is both a food and water source. A separate water sipper bottle may be provided, but may not be consumed.

喂養說明
建議在流食飼管中喂食流食，因為標準的吸水瓶可以、被顆粒物堵塞。要填充液體飼料飼管，請將拇指放在飼料槽上并填充從頂部流質飲食。將拇指放在進料口上，同時擰緊蓋子并倒置管釋放任何被困的氣泡。放置在籠子里的飼管架上。
準備好的飲食應冷藏保存，并應在 3 天內分發。
準備好的日糧在室溫下保存時間不應超過 48 小時，以防止日糧變質。
如果前一天已經準備好飲食，在分配前將瓶子倒置幾??次以重新懸浮可能已經沉降的顆粒。
普通小老鼠每天會消耗 20-30 毫升的流質飲食。平均大老鼠會消耗 80-100 毫升每天流質飲食。攝入量會因動物的大小和性別而異。
建議逐漸引入流質飲食。在存在以下情況時引入流質飲食
標準的固體飲食。在3-5天的時間內，增加流質飲食的量，同時減少量固體飲食，讓動物適應。含乙醇需要額外的馴化時間飲食。建議將動物逐漸引入乙醇飲食，

如下所示：
第 1-3 天 1/3 乙醇日糧和 2/3 對照日糧的混合飼料
第 4-7 天 飼料混合物 2/3 乙醇日糧和 1/3 對照日糧
第 8 天 僅飼喂乙醇飲食
流質飲食既是食物又是水源?？赡軙峁┮粋€單獨的吸水瓶，但可能不會被消耗。