日本 JaICA代理 Anti HEL monoclonal antibody

日本 JaICA 老化 Antibody系列產品

上海金畔生物作為日本 JaICA 老化中國代理商，歡迎新老客戶訪問日本 JaICA 老化官網或者咨詢我們獲取更多日本 JaICA 老化產品線價格說明書等信息。

日本老化制御研究所?Jaica代理

抗己酰-賴氨酸單克隆抗體 Anti Hexanoyl-Lysine(HEL) monoclonal antibody(5F12)

抗己酰-賴氨酸單克隆抗體 Anti Hexanoyl-Lysine(HEL) monoclonal antibody(5F12)
適用于免疫組織化學和ELISA。

Suitable for immunohistocheistry and ELISA. For research use only.

Oxidative damage of lipids caused by reactive oxygen species (ROS) play an important role in some diseases, lesion of cell functions and aging. Aldehydes such as malondi-aldehyde (MDA) and 4-hydroxy-2-nonenal (4-HNE) have been reported as one of the advanced lipid peroxidation products. But recently in the earlier stage of lipid peroxidation, 13-hydroperoxyoctadecanoic acid (13-HPODE) is found to be covalently bound to proteins¹⁾. Hexanoyl-Lysine adduct (HEL) is a novel lipid hydroperoxide-modified lysine residues. HEL is formed by oxidative modification by oxidized omega-6 fatty acids such as linoleic acid or arachidonic acid. This monoclonal antibody (clone 5H4)is spesific for proteins and peptides containing HEL. Suitable for HEL detection by immunohistochemistry and western blotting.

由活性氧（ROS）引起的脂質氧化損傷在一些疾病，細胞功能損害和衰老中起重要作用。醛類（如丙二醛/MDA）和4-羥基-2-壬烯醛（4-HNE）已被報道為先進的脂質過氧化產物之一，但最近在脂質過氧化的早期階段，發現13-hydroperoxyoctadecanoic acid(13-HPODE)與蛋白質1)共價結合。
己酰-賴氨酸加合物（HEL）是一種新的脂質氫過氧化物修飾的賴氨酸殘基， HEL通過氧化的omega-6脂肪酸（例如亞油酸或花生四烯酸）氧化修飾形成。
抗己酰-賴氨酸（HEL）單克隆抗體對于含有HEL的蛋白質和肽是特異的，適用于通過免疫組織化學和免疫印跡的HEL檢測。

A new biomarker for lipid peroxidation? ?脂質過氧化新的生物標志物

Cisplatin-treated rat kidney.?順鉑處理的大鼠腎臟
(Dr. Sugiyama, Tottori Univ.)??（Sugiyama博士）

Detection of HEL at Human atherosclerotic lesions.
(Dr. Naito)

HEL在人類動脈粥樣硬化病變的檢測??（Naito博士）

? 技術參數
克?。＃?F12；
亞基：IgG1 kappa；
抗原：N epsilon己酰-鑰孔血藍蛋白（HEL-KLH）；
特異性：特異于己酰-賴氨酸加合物；
形式：凍干粉，100μg/mL（200μL），PBS（pH7.4），包含5％蔗糖，1％ BSA；
應用：免疫組織化學（多聚甲醛固定的組織推薦抗體濃度為2μg/mL）和ELISA；
儲存：低于-20°C

參考文獻：

產品信息：

使用說明書
免疫組織化學方法
技術信息
克隆#5F12和5H4的比較

日本 JaICA老化Anti 4-HNE monoclonal antibody (HNEJ-2)

日本 JaICA 老化 Antibody系列產品

上海金畔生物作為日本 JaICA 老化中國代理商，歡迎新老客戶訪問日本 JaICA 老化官網或者咨詢我們獲取更多日本 JaICA 老化產品線價格說明書等信息。

日本老化制御研究所?Jaica代理

抗4-羥基壬烯醛單克隆抗體

Anti 4-hydroxy-2-nonenal(4-HNE) monoclonal antibody(HNEJ-2)
貨號：MHN-100P
品牌：JaICA
規格： 100μg

產品說明 / Product Overview：

Anti 4-HNE monoclonal antibody (HNEJ-2)

Structure of 4-hydroxy-2-nonenal

脂質氧化標志物

適用于免疫組織化學和免疫印跡.（僅供研究使用）

關于4-羥基壬烯醛（4-hydroxy-2-nonenal; 4-HNE）

活性氧（ROS）參與活體內的脂質過氧化，膜脂質是ROS的主要靶標之一。 ROS在過氧化過程中形成各種醛。 4-羥基-2-壬烯醛（4-HNE）是一種α，β不飽和醛，可以通過omega-6不飽和脂肪酸（例如亞油酸和花生四烯酸）的過氧化形成。 特別需要指出的是，據報道在活體內形成的4-HNE源自磷脂結合的花生四烯酸。 4-HNE可能是脂質過氧化最可靠的生物標志物之一。

大腸癌細胞的高倍視圖：腺癌（箭頭所示）和非腫瘤性上皮細胞(star x240)。
(S. Kondo et al. Free Radical Biology & Medicine 27 pp401-410, 1999)

技術參數

克隆號＃：HNEJ-2；
免疫原：4-HNE修飾的鑰孔血藍蛋白(KLH)；
亞基：小鼠IgG1（kappa）；制備為腹水，并純化硫酸銨；
性狀：凍干粉（含20μg或100μg IgG）；
特異性：該抗體對于用其它醛（如2-壬烯醛，2-己烯醛，1-己醛，4-羥基-2-己烯醛，甲醛或戊二醛）處理的蛋白質顯示出幾乎可以忽略的反應性；
應用：免疫組織化學和免疫印跡；
儲存：-20°C以下5年；運輸時，室溫下至少可穩定保持7天。

References

使用說明書
免疫組織化學操作方法
技術資料

日本 JaICA 老化Anti Acrolein (ACR) monoclonal antibody

上海金畔生物作為日本 JaICA 老化中國代理商，歡迎新老客戶訪問日本 JaICA 老化官網或者咨詢我們獲取更多日本 JaICA 老化產品線價格說明書等信息。

日本老化制御研究所?Jaica代理

抗丙烯醛單克隆抗體 Anti Acrolein(ACR) monoclonal antibody

Suitable for immunohistocheistry. For research use only.

Acrolein (ACR) is a representative carcinogenic aldehyde found ubiquitously in the environment and formed endogenously through oxidation reactions, such as lipid peroxidation and myeloperoxidasecatalyzed amino acid oxidation. ACR is highly reactive aldehyde and reacts with lysine residue in protein. The reaction with ACR and lysine residue leads to the formation of numerous numbers of adducts, such as formyl-dehydropiperidino-lysine (FDP-lysine) type derivative. This antibody is specific for the ACR-modified protein, especially FDP-lysine type derivative.

Specification

抗丙烯醛單克隆抗體適用于免疫組織化學。

丙烯醛（ACR）是在環境中普遍存在并且通過氧化反應（例如脂質過氧化和髓過氧化物酶催化的氨基酸氧化）內源性形成的代表性致癌醛。 ACR是高度反應性的醛，并與蛋白質中的賴氨酸殘基反應。與ACR和賴氨酸殘基的反應導致大量的加合物的形成，例如formyl-dehydropiperidino-lysine（FDP-賴氨酸）類型衍生物。
該抗體對ACR修飾的蛋白，特別是FDP-賴氨酸類型衍生物具有特異性。

技術參數
克?。＃?F6；
抗原：ACR修飾的鑰孔血藍蛋白；
形式：凍結（100μg/ mL抗體在含有0.1％NaN3和0.5％BSA的10mM PBS中）。通過蛋白A純化；
應用：免疫組織化學；
推薦抗體濃度：0.5-1.0μg/ mL對于多聚甲醛固定的組織；
特異性：特定針對ACR修飾的蛋白（特別是FDP-賴氨酸類型衍生物）；
亞型：小鼠IgG1，kappa；
儲存：小于-20°C

References

1)Protein-bound acrolein: Potential markers for oxidativestress. K.Uchida, M.Kanematsu, K.Sakai, T.Matsuda, N.Hattori,Y.Mizuno, D.Suzuki,T.Miyata, N.Noguchi, E.Niki,T.Osawa
Proc.Natl.Acad.Sci.USA,95,4882-4887(1998)

2)Protein-bound acrolein: A novel markers of oxidativestress in Alzheimer’s Disease. Noel Y. Calingasan, Koji Uchida, and Gary E.Gibson
Journal of Neurochemistry.72(2),751-756(1999)

日本 JaICA 老化Anti Acrolein monoclonal antibody

抗丙烯醛（ACR）單克隆抗體 使用使用說明書

技術資料

日本 JaICA 老化 Antibody系列產品

上海金畔生物作為日本 JaICA 老化中國代理商，歡迎新老客戶訪問日本 JaICA 老化官網或者咨詢我們獲取更多日本 JaICA 老化產品線價格說明書等信息。

日本老化制御研究所?Jaica代理

8-hydroxy-2′-deoxyguanosine (8-OHdG) is a product of oxidatively damaged DNA formed by hydroxy radical, singlet oxygen and direct photodynamic action. Anti 8-OHdG monoclonal antibody (clone N45.1) is highly specific for 8-OHdG, and is suitable for immunohistochemistry. Cross reactivity have been checked for 8-OHdG analogues (guanosine(G),7-methyl-G, 6-SH-G, 8-Bromo-G, dA, dC, dT, dI, dU, dG, O6-methyl-dG, 8-OHdA, guanine(Gua),O6-methyl-Gua, 8-OH-Gua, uric acid, urea, creatine, creatinine, 8-sulfhydryl-G, 8-OH-G).

Thymidineglycol (TG) is one of the major oxidation products of DNA. Thymidine (T) can be damaged by oxidative stress such as radiation and energy metabolism. Two different pathways to form TG have been suggested. Deoxythymidine in DNA is directly oxidised by hydroxy radical, to form TG. TG can be also formed through an intermediate thymidine chlorohydrin, which is derived from hypochlorous acid (HOCl) from neutrophil myeloperoxidase. Thymidineglycol is derived from DNA, not from RNA. TG is the oxidative stress marker secific for DNA damage.

Tyrosine is one of the major targets of protein oxidation, and until today various tyrosine derivatives such as nitrotyrosine, dityrosine and halogenated tyrosine depending on the type of free radicals. DT is a tyrosine dimer derived from tyrosyl radicals which is formed by reactive oxygen species (ROS), metal-catalyzed oxidation, ultraviolet irradiation, and peroxidases. DT have been found in atherosclerotic lesions, and lipofuscin of pyramidal neurons of aged human brains. Dityrosine is one of the specific biomarkers for protein oxidation.

Tyrosine is one of the main targets of protein oxidation, and it have been reported form nitrotyrosine, dityrosine and halogenated tyrosines depending on the type of free radicals. For example, hypochlorous acid (HOCl) and hypobromous acid (HOBr) are derived from neutrophil myeloperoxidase (MPO) and eosinophil peroxidase (EPO), and known to form dihalogenated tyrosines such as DiBrY, DiClY and DiIY. It is expected that DiBrY may be a novel biomarker for tissue damage which is related to inflammatory and allergic disorders.

During the peroxidation process, a variery of aldehydes are formed. 4-hydroxy-2-nonenal (4-HNE) is an alpha, beta unsatulated aldehyde that can be formed by peroxidation of omega-6 unsatulated fatty acids such as linoleic acid and arachidonic acid. Especially, 4-HNE formed inside living body is reported to originate from phospholipid-bound arachidonic acid. 4-HNE may be one of the most reliable biomarker of lipid peroxidation. This antibody show almost negligible reactivity with proteins that were treated with other aldehydes, such as 2-nonenal, 2-hexenal, 1-hexanal, 4-hydroxy-2-hexenal,formaldehyde, or glutaraldehyde.

Aldehydes such as malondi-aldehyde (MDA) and 4-hydroxy-2-nonenal (4-HNE) have been reported as one of the advanced lipid peroxidation products. But recently in the earlier stage of lipid peroxidation, 13-hydroperoxyoctadecanoic acid (13-HPODE) is found to be covalently bound to proteins1). Hexanoyl-Lysine adduct (HEL) is a novel lipid hydroperoxide-modified lysine residues. HEL is formed by oxidative modification by oxidized omega-6 fatty acids such as linoleic acid or arachidonic acid. This monoclonal antibody is spesific for proteins and peptides containing HEL. Suitable for HEL detection by immunohistochemistry and western blotting.

Acrolein (ACR) is a representative carcinogenic aldehyde found ubiquitously in the environment and formed endogenously through oxidation reactions, such as lipid peroxidation and myeloperoxidasecatalyzed amino acid oxidation. This antibody is specific for the ACR-modified protein, especially FDP-lysine type derivative.

Malondialdehyde (MDA) is one of the major aldehyde derive from lipid peroxidation. MDA is highly reactive aldehyde and reacts with lysine residue in protein. The reaction with MDA and lysine residue leads to the formation of numerous numbers of adducts, such as dihydropyridine-lysine (DHP-lysine) type derivative. This monoclonal antibody is specific for the MDA-modified protein, especially DHP-lysine type derivative.

4-hydroxy-2-alkenal is one of the major lipid peroxidation products, and shows many biological effects such as high toxicity to cells. Among them, 4-hydroxy-2-hexenal (HHE) is an aldehyde formed during peroxidation of n-3 fatty acids such as docosahexaenoic acid. HHE is highly reactive aldehyde and reacts with histidine residue of protein to form Michael-addition type adducts. This antibody is specific for HHE-histidine Michael adduct (HHE-His) and enable to detect HHE-His in the tissue samples.

Crotonaldehyde (CRA) is a representative carcinogenic aldehyde formed endogenously through lipid peroxidation. CRA is highly reactive aldehyde and reacts with lysine residue in protein. The reaction with CRA and lysine residue leads to the formation of numerous numbers of adducts. This antibody is specific for the CRA-modified protein.

Methylglyoxal (MG), an endogenous metabolite that increases in diabetes and is a common intermediate in the Maillard reaction (glycation), reacts with proteins and forms advanced glycation end products (AGE). MG reacts with arginine residue in protein and forms numerous numbers of adducts, such as argpyrimidine. This antibody is specific for argpyrimidine: N-(5-hydroxy-4,6-dimethylpyrimidine-2-yl)-L-ornithine.

Cholesterol oxidation products, especially 7-Ketocholesterol (7KC), have been the focus of much attention because they are present in human atherosclerotic plaque and display a wide range of atherogenic properties in vitro and to some extant in vivo. This antibody is specific for 7KC. This antibody is useful to detect 7KC in tissue samples. A frozen tissue sample is recommended for immunohistochemistry.

Reactive nitrogen species (RNS) are formed in a variety of diseases such as atherosclerosis, inflammation, cancer and neurodegenerative disiases. Peroxinitrite (ONOO^–) is known as a potent biological oxidant, which is formed via very fast reaction between nitric oxide (NO) and superoxide (O₂^–). 6-nitrotryptophan (6-NO²-Trp) is shown to be one of the most stable and major ONOO^–-modified amino acids, and growing evidences suggest that nitrotryptophan may play a significant role in the cellular regulation under nitrosative stress.

日本 JaICA 老化Anti Malondialdehyde(MDA) monoclonal antibody

中文名稱：抗丙二醛單克隆抗體

英文名稱：Anti Malondialdehyde(MDA) monoclonal antibody

貨號：MMD-030n

規格：30ug

Anti MDA monoclonal antibody? 脂質過氧化生物標志物

適用于免疫組化（僅供研究使用）

About malondialdehyde (MDA)

Malondialdehyde (MDA) is one of the major aldehyde derive from lipid peroxidation. MDA is highly reactive aldehyde and reacts with lysine residue in protein. The reaction with MDA and lysine residue leads to the formation of numerous numbers of adducts, such as dihydropyridine-lysine (DHP-lysine) type derivative. This monoclonal antibody is specific for the MDA-modified protein, especially DHP-lysine type derivative.

關于丙二醛（MDA）
丙二醛（MDA）是衍生自脂質過氧化的主要醛之一。 MDA是高度反應性的醛，并與蛋白質中的賴氨酸殘基反應。 與MDA和賴氨酸殘基的反應導致眾多數量的加合物的形成，例如二氫吡啶-賴氨酸（DHP-賴氨酸）型衍生物。 該單克隆抗體對MDA修飾的蛋白質，特別是DHP-賴氨酸型衍生物是特異性的。

Specifications

Specificity of anti MDA antibody.

Immunohistochemical detection of MDA-modified protein in atherosclerotic aorta.
Dr. N Shibata, Tokyo Women’s Medical University.

技術參數
克隆號＃：1F83；
抗原：MDA修飾的鑰孔血藍蛋白（KLH）；
性狀：凍結（100μg/mL抗體在含有0.1％NaN3和0.5％ BSA的10mM PBS中），經過蛋白A純化；
應用：免疫組織化學。對多聚甲醛固定的組織推薦抗體濃度為0.5-1.0μg/mL；
特異性：特異性針對MDA修飾的蛋白（特別是DHP-賴氨酸）。
亞基：小鼠IgG2a（lambda）
儲存：小于-20℃

其他相關產品：

·??抗丙烯醛單克隆抗體?Anti?Acrolein(ACR)?monoclonal?antibody

·??抗丙烯醛單克隆抗體?Anti?Acrolein(ACR)?monoclonal?antibody

·??抗己酰-賴氨酸單克隆抗體?Anti?Hexanoyl-Lysine(HEL)?monoclonal?antibody(5F12)

·??己酰-賴氨酸加合物ELISA試劑盒?Hexanoyl-Lysine?adduct(HEL)?ELISA?Kit

References 參考文獻

日本 JaICA 老化Anti Malondialdehyde(MDA) monoclonal antibody技術資料：http://www.jaica.com/e/technical_forum_nof.html#MDA

日本 JaICA 老化Anti Malondialdehyde(MDA) monoclonal antibody 說明書：

http://www.jaica.com/e/pdf/mda_ab_manual.pdf