日本JaICA Test kit for Potential Anti Oxidant (PAO)

日本JaICA Test kit for Potential Anti Oxidant (PAO)

日本老化制御研究所?Jaica代理

上海金畔生物作為日本 JaICA 老化中國代理商,歡迎新老客戶訪問日本 JaICA 老化官網或者咨詢我們獲取更多日本 JaICA 老化產品線價格說明書等信息。

Suitable for detection of total antioxidant capacity in serum and food extracts. For research use only.

Antioxidant assay:

Oxidative stress plays on important role in various diseases and aging. The control of oxidative stress is expected to be useful to prevent diseases and aging.Oxidative stress is caused by the imbalance between reactive oxygen species (ROS) and antioxidant defense system. For accurate assessment of oxidative stress, measurement of ROS, oxidative damage and antioxidant activity may be essential. Recently, antioxidants as functional foods which scavenge ROS attract a great deal of attention.

Principle of this assay:

In the PAO assay kit, an easy and convenient method to measure antioxidant capacity is provided. Utilizing the reduction of cupric ion (Cu++ to Cu+), antioxidant capacity of samples can be detected in 5 minutes. Samples are mixed with Cu++ Solution. Cu++ are reduced by antioxidants to form Cu+. Reduced Cu+ react with Chromatic Solution (Bathocuproine) , and can be detected by absorbance at wavelength 480 to 490 nm. Antioxidant capacity can be calculated from the Cu+ formed. PAO can detect not only hydrophilic antioxidants such as Vitamin C, glutathione, but also can detect hydrophobic antioxidants such as Vitamin E. Applicable for assessment of total antioxidants of serum, foods and beverage samples.

Specifications:

Method: Colormetric assay(detection: 480 – 492 nm)
Assay range: 21.9 – 4378 碌mol/L (cupric ion reducing power)
Format: 96 wells
Storage: Room temperature (10 – 25擄C)
Applications: Human and animal serum samples, foods and beverage samples.
Required but not provided: A micro plate reader (measuring wavelength 492 nm)
Pipettes and pipette chips
Plastic test tubes
Distilled water
NaOH, HCl solution and pH meter (Not required if standards are prepared with distilled water only).

 

Content of this kit: 試劑盒組成

Standard (Uric acid powder): 1 vial
Sample diluent: 1 bottle
Cu++?solution: 1 bottle
Stop solution: 1 bottle
Micro titer plate: 1 plate (96 wells)

Assay procedure:

1) Prepare 6 levels of standards by diluting 2mM uric acid.
2) Please prepare plastic test tubes for 6 levels of standards and each sample. Pour 390 碌L of Sample Diluent, and add 10 碌L of standards or diluted samples.
3) Pour 200 碌L of mixture to Micro titer plate. Use 200 碌L of Sample Diluent for blank well.
4) Read absorbance at 490 nm (as READ1).
5) Add 50 碌L of Cu++solution to each well, mix gently, and incubate at room temperature for 3 minutes.
6) Add 50 碌L of Stop solution, mix gently, and read absorbance at 490 nm (as READ2).
7) Please draw standard curves by plotting the difference of absorbance readings (READ2 – READ1) as vertical axis, and concentration of uric acid standards (mM) as horizontal axis. Calculate the corresponding uric acid concentration of samples. Multiply corresponding uric acid concentration (mM) of samples by 2189, to estimate antioxidant power (碌mol/L).
1mM of uric acid = 2189 碌mol/L (copper reducing power)

References

1) Oxidative imbalance and cathepsin D changes as peripheral blood biomarkers of Alzheimer disease: A pilot study
E Strafacea, P Matarresea, L Gambardella, R Vona, A Sgadari,MC Silveri, W Malorni
FEBS Letters 579, p2759-766 (2005)
2) Oxidative stress and its association with coronary artery disease and different atherogenic risk factors
C. VASSALLE, L. PETROZZI , N. BOTTO, M. G. ANDREASSI and G. C. ZUCCHELLI
Journal of Internal Medicine 256, p308-315(2004)
3) Antioxidant capacity as a reliable marker of stress in dairy calves transported by road
P Pregel, E Bollo, FT Cannizzo, B Biolatti, E Contato, and PG Biolatti
Veterinary Record 156, p53-54 (2005)
4) Vitamin E-coated dialyzers reduce oxidative stress related proteins and markers in hemodialysis ? a molecular biological approach.
LA Calo, A Naso, E Pagnin, PA Davis, M Castoro, R Corradin, P Riegler, C Cascone, W Huber and A Piccoli
Clinical Nephrology, Vol.62(5), p355-361 (2004)
5) Oxidative stress-related factors in Bartter’s and Gitelman9s syndrome: relevance for angiotensin IIsignalling.
Calo LA, Pagnin E, Davis PA, Sartori M, Semplicini A.
Nephrol Dial Transplant 18(8) p1518-1525 (2003)
6) Effect of epoetin on HO-1 mRNA level and plasma antioxidants in hemodialysis patients.
Calo LA, Stanic L, Davis PA, Pagnin E, Munaretto G, Fusaro M, Landini S, Semplicini A, Piccoli A.
Int. J Clin. Ther 41(5), p187-192 (2003)
7) Restored Antioxidant Capacity Parallels the Immunologic and Virologic Improvement in Children with Perinatal Human Immunodeficiency Virus Infection Receiving Highly Active Antiretroviral Therapy.
M Martino, F Chiarelli, M Moriondo, M Torello, C Azzari, and L Galli
Clinical Immunology 100(1),p82-86 (2001)
Product name Code Assay range Assay time Format
Test kit for Potential Anti Oxidant (PAO) KPA-050 21.9-4378 碌mol/L 5 minutes 96 wells

日本JaICA Test kit for Potential Anti Oxidant (PAO)? ?說明書? 技術資料

Instruction manual Technical information

日本Japan Institute for the Control of Aging (JaICA)

日本Japan Institute for the Control of Aging (JaICA)

日本老化制御研究所?Jaica代理

上海金畔生物作為日本 JaICA 老化中國代理商,歡迎新老客戶訪問日本 JaICA 老化官網或者咨詢我們獲取更多日本 JaICA 老化產品線價格說明書等信息。

獨特的氧化應激標記物

日本控制衰老研究所從事衰老研究,目的是促進人類健康。該研究所成功研發了檢測DNA氧化損傷的試劑盒,并且在這個領域處于領先地位。該研究所目前銷售很多關于氧化應激研究方面的產品。這些產品在醫學,藥學,生物化學和食品方面都有運用。該研究所也提供定制服務。隨著步入老齡化社會,預防醫學和抗衰老收到越來越多的關注。

JaICA從事試劑盒銷售和定制服務。試劑盒部分,研究所研發和生產用于檢測氧化應激標記物的試劑盒。單抗和ELISA試劑盒可用于檢測活性氧產生的氧化損傷產物。定制服務部分,使用本研究所研發的產品和積累的經驗檢測尿液和血液樣品,檢測食品。近來預防醫學、抗衰老和功能性食品的關注越來越多,因此全球醫院,大學,研究所、食品企業和制藥企業分析氧化應激標記物,衰老相關激素和礦物質需求也越來越多。

◆氧化應激標記物概況

 

類型 產品名稱 運用 備注
  尿液 血清 組織  
DNA?氧化 8-OHdG ? Check ELISA Kit 是(動物) 普遍使用的氧化應激標記物
檢測時間:大概3.5小時
8-OHdG ? Check ELISA Kit (High Sensitivity) 是(動物) 人血清、組織和培養細胞的更低檢測限。
anti-8-Hydroxy-2′-deoxyguanisine ? [8-OHdG],mAb(N45.1) 8-OHGua/8-OHG低交叉反應性
anti-Thymidine ? Glycol [TG], mAb (2E8) DNA特異性氧化應激標記物
脂類氧化 Hexanoyl-Lys ? [HEL] ELISA Kit n:6 多不飽和脂肪酸早期氧化的新標記物
anti-Hexanoyl-Lys ? [HEL], mAb (5F12) 免疫組化,免疫印跡和ELISA
anti-Hydroxy-2-nonenal ? [4-HNE], mAb(HNEJ-2) 普遍使用的脂類氧化標志物
anti-Acrolein ? [ACR], mAb (5F6) 免疫組化, 免疫印跡 和 ELISA.
anti-Malondialdehyde ? [MDA], mAb (1F83) 免疫組化 和 免疫印跡
anti-4-Hydroxy-2-hexenal ? [4-HHE], mAb (HHE53) 免疫組化
anti-Crotonaldehyde ? [CRA], mAb (82D3) 免疫組化
anti-Methylglyoxal ? [MG], mAb (3C) 免疫組化
anti-7-Ketocholesterol ? [7-KC], mAb (35A) 膽固醇氧化標記物
蛋白氧化 Dityrosine ? [DT] ELISA Kit 蛋白氧化新型標記物。檢測時間:過夜 + 1.5小時。
anti-Dityrosine ? [DT], mAb (1C3) 免疫組化, 免疫印跡 和 ELISA.
anti-Dibromo-tyrosine ? [DiBrY], mAb (3A5) 炎癥相關的蛋白氧化標記物
抗氧化檢測 Total ? Antioxidant Capacity [PAO] Test Kit Food
Samples
血清、食物和酒水總抗氧化檢測,能夠疏水和親水的抗氧化劑。

 

日本JaICA試劑盒產品目錄

日本JaICA試劑盒產品目錄

日本老化制御研究所?Jaica代理

上海金畔生物作為日本 JaICA 老化中國代理商,歡迎新老客戶訪問日本 JaICA 老化官網或者咨詢我們獲取更多日本 JaICA 老化產品線價格說明書等信息。

序號 詳細名稱 品牌 規格
KOG-200SE New 8-OHdG Check ELISA kit/8羥基脫氧蔦苷ELISA 試劑盒 JaICA 96 wells
KOG-200TE New 8-OHdG Check ELISA Trial Package (32wells) JaICA 32 wells
KOG-HS10E Highly Sensitive 8-OHdG Check ELISA kit/高靈敏?8羥基脫氧蔦苷ELISA 試劑盒 JaICA 96 wells
MOG-100P Anti 8-OHdG monoclonal antibody/8羥基脫氧蔦苷?單克隆抗體 JaICA 100 micro gram
MOG-020P Anti 8-OHdG monoclonal antibody/8羥基脫氧蔦苷?單克隆抗體 JaICA 20 micro gram
MHN-100P Anti 4-HNE monoclonal antibody/4羥基壬烯酸單抗 JaICA 100 micro gram
MHN-020P Anti 4-HNE monoclonal antibody/4羥基壬烯酸單抗 JaICA 20 micro gram
MBY-020P Anti Di bromo tyrosine (DiBrY) monoclonal antibody/二溴絡氨酸單抗 JaICA 20 micro gram
MTG-100P Anti Tymidine Glycol (TG) monoclonal antibody/抗胸苷乙二醇單克隆抗體 JaICA 100 micro gram
MDT-020P Anti Di Tyrosine (DT) monoclonal antibody/二絡氨酸抗體 JaICA 20 micro gram
MHL-021P Anti Hexanoyl-Lys(HEL) monoclonal antibody (clone 5F12)/乙酰賴氨酸單克隆抗 JaICA 20 micro gram
KHL-700E Hexanoyl-Lys(HEL) ELISA kit/乙酰賴氨酸加合物ELISA試劑盒 JaICA 96 wells
KPA-050E Test kit for Potential Anti Oxidant (PAO)/總抗氧化能力ELISA試劑盒 JaICA 96 wells
MMD-030n Anti malondialdehyde (MDA) monoclonal antibody/丙二醛單克隆 JaICA 30 micro gram
MAR-020n Anti acrolein (ACR) monoclonal antibody/炳烯醛單克隆 JaICA 20 micro gram
MAR-100n Anti acrolein (ACR) monoclonal antibody/炳烯醛單克隆 JaICA 100 micro gram
MHH-030n Anti 4-hydroxy-2-hexenal (4-HHE) monoclonal antibody/4羥基?2烯醛單抗 JaICA 30 micro gram
MCA-030n Anti crotonaldehyde (CRA) monoclonal antibody/抗巴斗醛單抗 JaICA 30 micro gram
MMG-030n Anti methyglyoxal (MG) monoclonal antibody/甲基乙二醛單抗 JaICA 30 micro gram
MKC-020n Anti 7-ketocholesterol (7-KC) monoclonal antibody/7酮基膽固醇單抗 JaICA 20 micro gram
MKC-100n Anti 7-ketocholesterol (7-KC) monoclonal antibody/7酮基膽固醇單抗 JaICA 100 micro gram
OPF-010E NEW Ultra filter unit for serum pretreatment (MW cut off 10kDa, Pall Corp.)/血清預處理超濾裝置(MW切斷10kDa,Pall公司) JaICA 1 unit
KDT-010E Dityrosine (DT) ELISA/雙絡氨酸ELISA試劑盒 JaICA ?96 wells

 

日本 JaICA代理 Anti Di Tyrosine (DT) 單抗

日本 JaICA代理 Anti Di Tyrosine (DT) 單抗

日本老化制御研究所自成立以來就對抗衰老進行研究,它是在有助于健康促進的理念下創建的。結果,此研究所在開發DNA氧化損傷測試試劑盒方面取得了很大的成功,并在該領域處于了領先地位。

本研究所現在銷售許多氧化應激相關產品,這些產品可應用于醫學,藥學,生化學,食品科學等。它還利用其獨特的技術開展檢測服務。隨著社會老齡化的發展,預防醫學與抗衰老引起了越來越多的關注,在這些領域中,我們研究所將會通過開發和提供研究產品來助于營造一個健康,長壽的社會。

產品類型:

DNA氧化檢測產品;

蛋白氧化檢測產品;

脂肪氧化檢測產品;

抗氧化檢測產品。

特性:

高專一性、高靈敏性;

不與RNA損傷產生交叉反應;

易于操作、簡單快速;

無需昂貴的設備和樣品預處理。

上海金畔生物作為日本 JaICA 老化中國代理商,歡迎新老客戶訪問日本 JaICA 老化官網或者咨詢我們獲取更多日本 JaICA 老化產品線價格說明書等信息。

日本老化制御研究所?Jaica代理

日本 JaICA代理 Anti Di Tyrosine (DT) 單抗

產品名稱:?Anti Di Tyrosine (DT) 單抗

英文名稱:?Anti Di Tyrosine (DT) monoclonal antibody

產品貨號:?MDT-020P

產品規格:?20ug

產品產地:?日本

產品商標:?JaICA

 

Code Product Name  
KOG-200SE New 8-OHdG Check ELISA kit 96 wells
KOG-200TE New 8-OHdG Check ELISA Trial Package (32wells) 32 wells
KOG-HS10E Highly Sensitive 8-OHdG Check ELISA kit 96 wells
MOG-100P Anti 8-OHdG monoclonal antibody 100 micro gram
MOG-020P Anti 8-OHdG monoclonal antibody 20 micro gram
MHN-100P Anti 4-HNE monoclonal antibody 100 micro gram
MHN-020P Anti 4-HNE monoclonal antibody 20 micro gram
MBY-020P Anti Di bromo tyrosine (DiBrY) monoclonal antibody 20 micro gram
MTG-100P Anti Tymidine Glycol (TG) monoclonal antibody 100 micro gram
MDT-020P Anti Di Tyrosine (DT) monoclonal antibody 20 micro gram
MHL-021P Anti Hexanoyl-Lys(HEL) monoclonal antibody (clone 5F12) 20 micro gram
KHL-700E Hexanoyl-Lys(HEL) ELISA kit 96 wells
KPA-050 Test kit for Potential Anti Oxidant (PAO) 96 wells
MMD-030n Anti malondialdehyde (MDA) monoclonal antibody 30 micro gram
MAR-020n Anti acrolein (ACR) monoclonal antibody 20 micro gram
MAR-100n Anti acrolein (ACR) monoclonal antibody 100 micro gram
MHH-030n Anti 4-hydroxy-2-hexenal (4-HHE) monoclonal antibody 30 micro gram
MCA-030n Anti crotonaldehyde (CRA) monoclonal antibody 30 micro gram
MMG-030n Anti methyglyoxal (MG) monoclonal antibody 30 micro gram
MKC-020n Anti 7-ketocholesterol (7-KC) monoclonal antibody 20 micro gram
OPF-010E NEW Ultra filter unit for serum pretreatment (MW cut off 10kDa, Pall Corp.) 1 unit
KDT-010E Dityrosine (DT) ELISA ?96 wells
OPNUCP1 Nuclease P1 from Penicillium citrinum 500 units

 

日本 JaICA代理 Dityrosine (DT) ELISA

日本 JaICA代理 Dityrosine (DT) ELISA

日本 JaICA 老化 Antibody系列產品

上海金畔生物作為日本 JaICA 老化中國代理商,歡迎新老客戶訪問日本 JaICA 老化官網或者咨詢我們獲取更多日本 JaICA 老化產品線價格說明書等信息。

日本老化制御研究所?Jaica代理

Dityrosine (DT) ELISA kit.

Suitable for urine samples. For research use only.

A novel biomarker for protein oxidation

Tyrosine is one of the major targets of protein oxidation, and until today various tyrosine derivatives such as nitrotyrosine, dityrosine and halogenated tyrosine depending on the type of free radicals. DT is a tyrosine dimer derived from tyrosyl radicals which is formed by reactive oxygen species (ROS), metal-catalyzed oxidation, ultraviolet irradiation, and peroxidases. DT have been found in atherosclerotic lesions, and lipofuscin of pyramidal neurons of aged human brains. Dityrosine is one of the specific biomarkers for protein oxidation.

Recently, dityrosine is reported to exist also in urine samples. It is expected that DT may be a novel protein oxdation marker, which is non-invasively detectable. DT ELISA kit is designed for quantitative measurement of DT especially in urine samples.

試劑盒組成:

Samples: Urine
Specifity: Specific to dityrosine (tyrosine dimer)
Assay range: 0.05 – 12 碌mol/L
Measurement: 450 nm
Quantity: 96 wells
Assay time: Overnight and 2 hours
Storage: 2 – 8 擄C. Don’t freeze.
Expiration: Printed on a package

參考文獻:

1) Kato Y, Wu X, Naito M, Nomura H, Kitamoto N, Osawa T:
Immunochemical detection of protein dityrosine in atherosclerotic lesion of apo-E-deficient mice using a novel monoclonal antibody.
Biochem Biophys Res Commun. 275(1), p11-15 (2000).
2) Hattori Y, Mukaide T, Jiang L, Kotani T, Tsuda H, Mano Y, Sumigama S, Hirayama T, Nagasawa H,Kikkawa F, Toyokuni S:
Catalytic ferrous iron in amniotic fluid as a predictive marker of human maternal-fetal disorders.
J Clin Biochem Nutr 56(1),p57-63(2015) doi: 10.3164/jcbn.14-82
Product name Code Assay range Format Application
Dityrosine (DT) ELISA KDT-010E 0.05 – 12 碌mol/L 96 wells Urine

使用說明書:

Instruction manual (PDF) Technical information

相關產品:

Related products: Anti DT monoclonal antibody

日本JaICA 代理Hexanoyl-Lysine adduct(HEL) ELISA Kit

日本JaICA 代理Hexanoyl-Lysine adduct(HEL) ELISA Kit

Hexanoyl-lysine adduct (HEL) ELISA kit

Suitable for assessment of oxidative stress using urine, serum and cultured cells. For research use only.

已酰-賴氨酸(HEL)ELISA試劑盒,適用于尿液,血清和細胞培養樣品氧化應激的評估。
僅供研究使用。

什么是己酰-賴氨酸?

Biomarker for early stage of lipid oxidation

Oxidative damage of lipids caused by reactive oxygen species (ROS) play an important role in some diseases, lesion of cell functions and aging. Aldehydes such as malondi-aldehyde (MDA) and 4-hydroxy-2-nonenal (4-HNE) have been reported as one of the advanced lipid peroxidation products. But recently in the earlier stage of lipid peroxidation, 13-hydroperoxyoctadecanoic acid (13-HPODE) is found to be covalently bound to proteins1). Hexanoyl-Lysine adduct (HEL) is a novel lipid hydroperoxide-modified lysine residues. HEL is formed by oxidative modification by oxidized omega-6 fatty acids such as linoleic acid or arachidonic acid. HEL may be a useful biomarker for initial stage of lipid peroxidation.Monoclonal antibodies and ELISA kit have been developped, and HEL can be detected in oxidatively modified LDL, in human atherosclerotic lesions, human urine and serum. It is also reported that HEL is formed in rat muscle during exercise, and the formation is prohibited by antioxidants such as flavonoids.

由活性氧(ROS)引起的脂質氧化損傷在一些疾病、細胞功能損害和衰老中起重要作用。醛類如丙二醛(MDA)和4-羥基-2-壬烯醛(4-HNE)已被報道為先進的脂質過氧化產物之一。但最近在脂質過氧化的早期階段,發現13-氫過氧十八烷酸(13-HPODE)與蛋白質共價結合。己酰-賴氨酸加合物(HEL)是一種新的脂質氫過氧化物修飾的賴氨酸殘基。HEL是通過氧化的omega-6脂肪酸(例如亞油酸或花生四烯酸)的氧化修飾形成。HEL可能是脂質過氧化初始階段有用的生物標志物?,F在我們已經開發了HEL單克隆抗體和ELISA試劑盒,并且可以在氧化修飾的低密度脂蛋白(LDL)、人動脈粥樣硬化病變、人尿液和血清中檢測HEL。另據報道,大鼠肌肉在運動期間形成HEL,并且通過抗氧化劑(如類黃酮)是禁止形成的。

己酰-賴氨酸(HEL)ELISA試劑盒

JaICA have developed HEL ELISA kit in collaboration with Dr. Toshihiko Osawa (Nagoya University) and Dr. Yoji Kato (Univerisity of Hyogo). This ELISA kit can be applied to urine, serum and cultured cells form human and animal.

JaICA公司與Toshihiko Osawa博士(名古屋大學)和Yoji Kato博士(兵庫大學)合作開發了Hexanoyl-Lys(HEL)ELISA試劑盒。
該ELISA試劑盒可應用于人和動物的尿液、血清和細胞培養。

Assay principle: Competitive ELISA (detection: 450 nm)
Specifity: Specific to N-epsilon-Hexanoyl-Lysine adduct
Measuring range: 2 – 700 nmol/L
Time to answer: Over night and 2 hours.
Format: 96 wells (54 samples in single assay)
Applications: Urine, serum and cultured cells from human and animals.
Storage: Store at 2 – 8擄C (don’t freeze).
Expiry: 2 years after the day of manufacturing.
Required but not provided: 50 碌L micropipettor and pipette tips
8-channel (50-200 碌L) micropipettor and tips
8 or 12-syncronous multichannel pipet and reagent tray for multichannel pipet.
4-7 擄C incubator
Microtiter plate reader (measuring wavelength 450 nm)

技術參數
檢測原理:競爭法ELISA (檢測: 450 nm處);
特異性:特異于N-epsilon-己酰-賴氨酸加合物;
測定范圍:2 – 700 nmol/L;
反應速度:過夜+2小時;
規格:96孔(54 samples in single assay);
應用:人和動物的尿液、血清和細胞培養物;
儲存:2-8℃(避免冷凍);
保質期:2年。

Content of this kit

HEL-coated Microtiter Plate: 1 plate (96 wells)
Primary Antibody (ready to use): 1 vial
Secondary Antibody: 1 vial
Secondary Antibody Buffer: 1 vial
Chromogen (TMBZ solution): 1 vial
Chromogen Buffer: 1 vial
Washing Buffer (5X): 1 vial
Stop Solution: 1 vial
Standard solution (6 levels): 1 vial each
Plate seal: 2 sheets

試劑盒組成:

HEL-包被微孔板: 1 塊板(96孔)
一抗 (即用型): 1?瓶
二抗: 1?瓶
二抗緩沖液: 1?瓶
底物溶液 (TMBZ溶液): 1?瓶
底物緩沖液: 1 瓶
洗滌液 (5X): 1 瓶
終止液: 1 瓶
標準液 (6 標準): 各1 瓶
封板膜: 2 片

Product name Code Assay range Application
Hexanoyl-Lys (HEL) ELISA KHL-700E 2-700 nmol/L Urine, serum, cultured cells and other biological materials

參考文獻:

1)
Yoji Kato, Yoko Mori, Yuko Makino, Yasujiro Morimitsu, Sadayuki Hiroi, Toshitsugu Ishikawa, Toshihiko Osawa, Formation of N epsilon-(hexanonyl) lysine in protein exposed to lipid hydroperoxide. J Biol Chem 274(29), p20406-20414 (1999)
Identification of HEL, which is lysine adduct of 13-HPODE.
2)
Yoji Kato, Toshihiko Osawa: Detection of lipid hydroperoxide-derived protein modification with polyclonal antibodies. Methods in Molecular Biology, vol. 186, Oxidative stress biomarkers and antioxidant protocols, D Armstrong, Ed., Human Press Inc., NJ, USA, p37-44
Immunohistochemical detection of hexanoyl-lysine adduct.
3)
Kato Y, Miyake Y, Yamamoto K, Shimomura Y, Ochi H, Mori Y, Osawa T, Preparation of a monoclonal antibody to N(epsilon)-(Hexanonyl)lysine: application to the evaluation of protective effects of flavonoid supplementation against exercise-induced oxidative stress in rat skeletal muscle. Biochem Biophys Res Commun 274(2),p389-393(2000)
Development and characterization of anti HEL monoclonal antibody.
4)
Ryo K, Yamada H, Nakagawa Y, Tai Y, Obara K, Inoue H, Mishima K, Saito I, Possible involvement of oxidative stress in salivary gland of patients with Sjogren’s syndrome. Pathobiology 73(5), p252-260 (2006)
HEL can be detected in saliva samples from patients with Sjogren’s syndrome.
5)
Suzuki T, Kazui T, Yamamoto S, Washiyama N, Ohkura K, Ohishi K, Bashar AH, Yamashita K, Terada H, Suzuki K, Akuzawa S, Fujie M, Effect of prophylactically administered edaravone during antegrade cerebral perfusion in a canine model of old cerebral infarction. J Thorac Cardiovasc Surg 133(3),p710-716 (2007)
Application to canine serum samples.
6)
Shimizu K, Ogawa F, Akiyama Y, Muroi E, Yoshizaki A, Iwata Y, Komura K, Bae S, Sato S, Increased Serum Levels of N(epsilon)-(hexanoyl)lysine, A New Marker of Oxidative Stress, in Systemic Sclerosis. J Rheumatol. 2008 Sep 1.
Serum HEL concentration from systemic sclerosis is higher than that from healthy subjects.
7)
Tamura H, Takasaki A, Miwa I, Taniguchi K, Maekawa R, Asada H, Taketani T, Matsuoka A, Yamagata Y, Shimamura K, Morioka H, Ishikawa H, Reiter RJ, Sugino N, Oxidative stress impairs oocyte quality and melatonin protects oocytes from free radical damage and improves fertilization rate. J Pineal Res 44(3),p280-287(2008)
Intrafollicular concentration of HEL was significantly reduced by these antioxidant treatment.
8)
Sakamoto Y, Ishikawa T, Kondo Y, Yamaguchi K, Fujisawa M, The assessment of oxidative stress in infertile patients with varicocele. BJU Int 101(12), p1547-1552 (2008)
Azoospermic and oligospermic patients had a significantly higher HEL concentration in seminal plasma.
9)
Kageyama Y, Takahashi M, Nagafusa T, Torikai E, Nagano A, Etanercept reduces the oxidative stress marker levels in patients with rheumatoid arthritis. Rheumatol Int 28(3),pp245-251(2008)
Urinary HEL level was reduced at 3 and 6 months after the initial treatment with etanercept.
10)
Rummenie VT, Matsumoto Y, Dogru M, Wang Y, Hu Y, Ward SK, Igarashi A, Wakamatsu T, Ibrahim O, Goto E, Luyten G, Inoue H, Saito I, Shimazaki J, Tsubota K, Tear cytokine and ocular surface alterations following brief passive cigarette smoke exposure. Cytokine 43(2),p200-208(2008)
HEL concentration in tear was increased by brief passive exposure to cigarette smoke.
11)
K Sakai, S Kino, A Masuda, M Takeuchi, T Ochi, J Osredkar, B Rejc, K Gersak, N Ramarathnam, Y Kato;
Determination of HEL (Hexanoyl-Lysine Adduct): A Novel Biomarker for Omega-6 PUFA Oxidation.
Lipid Hydroperoxide-Derived Modification of Biomolecules: Subcellular Biochemistry 77,p61-72(2014)
An original paper for HEL ELISA development.

  • 尿8-異前列腺素(15-異前列腺素F2t)ELISA試劑盒?NWLSS™?Urinary?8-Isoprostane
  • 脂質氫過氧化物檢測試劑盒?NWLSS™?Lipid?Hydroperoxide(LOOH)?Assay?Kit
  • 8-異構前列腺素(15-異前列腺素F2t)ELISA試劑盒?NWLSS™?8-Isoprostane?(15-Iso
  • 9(±)-羥基十八碳二烯酸ELISA試劑盒?NWLSS™?9(±)-hydroxyoctadecadienoic?ac
  • 己酰賴氨酸ELISA試劑盒?NWLSS™?Hexanoyl?Lysine?ELISA?Kit
  • 丙二醛檢測試劑盒?NWLSS™?Malondialdehyde(MDA)?Assay?Kit
  • 抗4-羥基壬烯醛單克隆抗體?Anti?4-hydroxy-2-nonenal(4-HNE)?monoclonal?antibody(HNE
  • 抗4-羥基壬烯醛單克隆抗體?Anti?4-hydroxy-2-nonenal(4-HNE)?monoclonal?antibody(HNE

使用手冊????????質量控制??????? 技術資料

Instruction manual Quality control Technical information

日本老化 JaICA品牌 單克隆抗體-JaICA

日本老化 JaICA品牌 單克隆抗體-JaICA

KOG-200SE New?8-OHdG?Check?ELISA?kit 96?wells
KOG-200TE New?8-OHdG?Check?ELISA?Trial?Package?(32wells) 32?wells
KOG-HS10E Highly?Sensitive?8-OHdG?Check?ELISA?kit 96?wells
MOG-100P Anti?8-OHdG?monoclonal?antibody 100?micro?gram
MOG-020P Anti?8-OHdG?monoclonal?antibody 20?micro?gram
MHN-100P Anti?4-HNE?monoclonal?antibody 100?micro?gram
MHN-020P Anti?4-HNE?monoclonal?antibody 20?micro?gram
MBY-020P Anti?Di?bromo?tyrosine?(DiBrY)?monoclonal?antibody 20?micro?gram
MTG-100P Anti?Tymidine?Glycol?(TG)?monoclonal?antibody 100?micro?gram
MDT-020P Anti?Di?Tyrosine?(DT)?monoclonal?antibody 20?micro?gram
MHL-021P Anti?Hexanoyl-Lys(HEL)?monoclonal?antibody?(clone?5F12) 20?micro?gram
KHL-700E Hexanoyl-Lys(HEL)?ELISA?kit 96?wells
KPA-050 Test?kit?for?Potential?Anti?Oxidant?(PAO) 96?wells
MMD-030n Anti?malondialdehyde?(MDA)?monoclonal?antibody 30?micro?gram
MAR-020n Anti?acrolein?(ACR)?monoclonal?antibody 20?micro?gram
MAR-100n Anti?acrolein?(ACR)?monoclonal?antibody 100?micro?gram
MHH-030n Anti?4-hydroxy-2-hexenal?(4-HHE)?monoclonal?antibody 30?micro?gram
MCA-030n Anti?crotonaldehyde?(CRA)?monoclonal?antibody 30?micro?gram
MMG-030n Anti?methyglyoxal?(MG)?monoclonal?antibody 30?micro?gram
MKC-020n Anti?7-ketocholesterol?(7-KC)?monoclonal?antibody 20?micro?gram
MKC-100n Anti?7-ketocholesterol?(7-KC)?monoclonal?antibody 100?micro?gram
OPF-010E?NEW Ultra?filter?unit?for?serum?pretreatment?(MW?cut?off?10kDa,?Pall?Corp.) 1?unit
KDT-010E Dityrosine?(DT)?ELISA ?96?wells
OPNUCP1 Nuclease?P1?from?Penicillium?citrinum 500?units

日本JaICA代理 KDT-010E Dityrosine (DT) ELISA/雙絡氨酸ELISA試劑盒

日本JaICA代理 KDT-010E Dityrosine (DT) ELISA/雙絡氨酸ELISA試劑盒
日本老化制御研究所 Jaica代理
日本Japan Institute for the Control of Aging (JaICA)
上海金畔生物代理日本 JaICA 老化品牌產品,歡迎新老客戶訪問日本 JaICA 老化官網或者咨詢我們獲取更多日本 JaICA 老化產品線價格說明書等信息。

日本JaICA代理 MKC-100n Anti 7-ketocholesterol (7-KC) monoclonal antibody/7酮基膽固醇單抗

日本JaICA代理 MKC-100n Anti 7-ketocholesterol (7-KC) monoclonal antibody/7酮基膽固醇單抗
日本老化制御研究所 Jaica代理
日本Japan Institute for the Control of Aging (JaICA)
上海金畔生物代理日本 JaICA 老化品牌產品,歡迎新老客戶訪問日本 JaICA 老化官網或者咨詢我們獲取更多日本 JaICA 老化產品線價格說明書等信息。

日本老化JaICA代理及JaICA品牌介紹

日本老化JaICA代理及JaICA品牌介紹

Japan Institute for the Control of Aging (JaICA)

上海金畔生物代理日本 JaICA 老化品牌產品,歡迎新老客戶訪問日本 JaICA 老化官網或者咨詢我們獲取更多日本 JaICA 老化產品線價格說明書等信息。

日本老化制御研究所?Jaica代理

日本老化制御研究所自成立以來就對抗衰老進行研究,它是在有助于健康促進的理念下創建的。結果,此研究所在開發DNA氧化損傷測試試劑盒方面取得了很大的成功,并在該領域處于了地位。

本研究所現在銷售許多氧化應激相關產品,這些產品可應用于醫學,藥學,生化學,食品科學等。它還利用其獨特的技術開展檢測服務。隨著社會老齡化的發展,預防醫學與抗衰老引起了越來越多的關注,在這些領域中,我們研究所將會通過開發和提供研究產品來助于營造一個健康,長壽的社會。

產品類型:

1. DNA氧化檢測產品;

2. 蛋白氧化檢測產品;

3. 脂肪氧化檢測產品;

4. 抗氧化檢測產品。

特性:

1. 高專一性、高靈敏性;

2. 不與RNA損傷產生交叉反應;

3. 易于操作、簡單快速;

4. 無需昂貴的設備和樣品預處理。

日本Jaica

The Japan Institute for the Control of Aging has conducted research into control of aging since its foundation under the doctrine of contributing health promotion. As a result, the institute succeeded in developing a DNA oxidative damage measuring kit, thus taking the lead for this field.
The institute now sells many products related to oxidative stress, and such products are utilized in the fields of medicine, pharmacy, biochemistry, food sciences, etc. The institute also performs assay services utilizing its unique technologies. Preventive medicine and anti-aging are receiving increased attention with the advancement of aging society. In these areas, our Institute will contribute to realizing a healthy, long-lived society by developing and supplying research products.

獨特的氧化應激標記物

日本控制衰老研究所從事衰老研究,目的是促進人類健康。該研究所成功研發了檢測DNA氧化損傷的試劑盒,并且在這個領域處于領先地位。該研究所目前銷售很多關于氧化應激研究方面的產品。這些產品在醫學,藥學,生物化學和食品方面都有運用。該研究所也提供定制服務。隨著步入老齡化社會,預防醫學和抗衰老收到越來越多的關注。

JaICA從事試劑盒銷售和定制服務。試劑盒部分,研究所研發和生產用于檢測氧化應激標記物的試劑盒。單抗和ELISA試劑盒可用于檢測活性氧產生的氧化損傷產物。定制服務部分,使用本研究所研發的產品和積累的經驗檢測尿液和血液樣品,檢測食品。近來預防醫學、抗衰老和功能性食品的關注越來越多,因此全球醫院,大學,研究所、食品企業和制藥企業分析氧化應激標記物,衰老相關激素和礦物質需求也越來越多。

◆氧化應激標記物概況

類型 產品名稱 運用 備注
尿液 血清 組織
DNA?氧化 8-OHdG ? Check ELISA Kit 是(動物) 普遍使用的氧化應激標記物
檢測時間:大概3.5小時
8-OHdG ? Check ELISA Kit (High Sensitivity) 是(動物) 人血清、組織和培養細胞的更低檢測限。
anti-8-Hydroxy-2′-deoxyguanisine ? [8-OHdG],mAb(N45.1) 8-OHGua/8-OHG低交叉反應性
anti-Thymidine ? Glycol [TG], mAb (2E8) DNA特異性氧化應激標記物
脂類氧化 Hexanoyl-Lys ? [HEL] ELISA Kit n:6 多不飽和脂肪酸早期氧化的新標記物
anti-Hexanoyl-Lys ? [HEL], mAb (5F12) 免疫組化,免疫印跡和ELISA
anti-Hydroxy-2-nonenal ? [4-HNE], mAb(HNEJ-2) 普遍使用的脂類氧化標志物
anti-Acrolein ? [ACR], mAb (5F6) 免疫組化, 免疫印跡 和 ELISA.
anti-Malondialdehyde ? [MDA], mAb (1F83) 免疫組化 和 免疫印跡
anti-4-Hydroxy-2-hexenal ? [4-HHE], mAb (HHE53) 免疫組化
anti-Crotonaldehyde ? [CRA], mAb (82D3) 免疫組化
anti-Methylglyoxal ? [MG], mAb (3C) 免疫組化
anti-7-Ketocholesterol ? [7-KC], mAb (35A) 膽固醇氧化標記物
蛋白氧化 Dityrosine ? [DT] ELISA Kit 蛋白氧化新型標記物。檢測時間:過夜 + 1.5小時。
anti-Dityrosine ? [DT], mAb (1C3) 免疫組化, 免疫印跡 和 ELISA.
anti-Dibromo-tyrosine ? [DiBrY], mAb (3A5) 炎癥相關的蛋白氧化標記物
抗氧化檢測 Total ? Antioxidant Capacity [PAO] Test Kit Food
Samples
血清、食物和酒水總抗氧化檢測,能夠疏水和親水的抗氧化劑。